mutL

168

sequence-unspecific endonuclease, DNA mismatch repair

locus: BSU_17050

Molecular weight: 70.25 kDa

pI: 5.58

Protein length: 627 aa

Gene length: 1884 bp

function: DNA repair

product: DNA mismatch repair protein, sequence-unspecific endonuclease

essential: no

synonyms: mutL

List of homologs in different organisms, belongs to COG0323 (Galperin et al., 2021)

Gene

Coordinates: 1,778,337  1,780,220

Phenotypes of a mutant

• 60-fold increased mutation rate [Pubmed|23882084]
• a ''[gene|D5275ECDC2A70FBAC2CA5980F04B272C3328FC90|rnhB] [gene|E2A8B04CD729332F9E6A13195181983F751F2F25|mutS]-[gene|A86A013EDCE59D7EF84FB836A751E66C58CADF45|mutL]'' mutant is not viable [Pubmed|23882084]

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The protein

Protein family

• DNA mismatch repair mutL/hexB family (single member, according to UniProt)

Structure

• [PDB|3KDK] (C-terminal domain) [Pubmed|20603082]
• [PDB|6E8D] (complexed with [protein|83E05071DEC874248D3E96B8F3A093C65939B314|dnaN]) [Pubmed|30916336]
• [AF|P49850]

[wiki|Localization]

• forms foci at midcell position, the frequency of foci increases upon mismatch formation [Pubmed|21958350]

Expression and Regulation

Operons

• genes: [gene|E2A8B04CD729332F9E6A13195181983F751F2F25|mutS]-[gene|A86A013EDCE59D7EF84FB836A751E66C58CADF45|mutL]

  description: [Pubmed|8760914]

  additional information

  • A [protein|search|ncRNA] is predicted between '[protein|search|mutL]' and '[protein|search|ymzD]' [PubMed|20525796]

Biological materials

Mutant

• GP1190 ([gene|E2A8B04CD729332F9E6A13195181983F751F2F25|mutS]-[gene|A86A013EDCE59D7EF84FB836A751E66C58CADF45|mutL]::''aphA3''), available in [wiki|Jörg Stülke]'s lab [Pubmed|22178973]
• 1A833 ( ''mutL''::''spec''), [Pubmed|11779496], available at [http://bgsc.org/search.php?Search=1A833 Bacillus Genetic Stock Center]
• BKE17050 ([gene|A86A013EDCE59D7EF84FB836A751E66C58CADF45|mutL]::erm  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE17050 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_TTTTGCCACATTCATCACCC,  downstream forward: _UP4_TAGCGGGGGTGGTAGGCATT
• BKK17050 ([gene|A86A013EDCE59D7EF84FB836A751E66C58CADF45|mutL]::kan  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK17050 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_TTTTGCCACATTCATCACCC,  downstream forward: _UP4_TAGCGGGGGTGGTAGGCATT

References

Reviews

• 22933559,26354434,26343983,35210609,38551349

Original Publications

• 8760914,15375129,20603082,20525796,22843852,21050827,21958350,22178973,23882084,23998896,25326311,26384423,26163658,27369079,27851738,30391220,30916336,35670670,35847079