murD

168

UDP-N-acetylmuramoyl-L-alanyl-D-glutamate synthetase

locus: BSU_15200

Molecular weight: 49.49 kDa

pI: 5.57

Protein length: 451 aa

Gene length: 1356 bp

function: peptidoglycan precursor biosynthesis

product: UDP-N-acetylmuramoyl-L-alanyl-D-glutamate synthetase

essential: yes

ec: 6.3.2.9

synonyms: murD

List of homologs in different organisms, belongs to COG0771 (Galperin et al., 2021)

Gene

Coordinates: 1,588,901  1,590,256

Phenotypes of a mutant

• essential [Pubmed|12682299]

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The protein

Catalyzed reaction/ biological activity

• ATP + D-glutamate + UDP-N-acetyl-α-D-muramoyl-L-alanine --> ADP + H⁺ + phosphate + UDP-N-acetyl-α-D-muramoyl-L-alanyl-D-glutamate (according to UniProt)

Protein family

• [wiki|MurCDEF family] (according to UniProt)

Structure

• [PDB|3LK7] (from Streptococcus agalactiae, 50% identity)
• [AF|Q03522]

[wiki|Localization]

• cytoplasm (according to Swiss-Prot)

Expression and Regulation

Operons

• genes: [gene|D48B7591467C493D2214D37AEA83643FE8C27BC6|murE]-[gene|4D3D4429E1F387BACB0D5A1084AA8DF179A16685|mraY]-[gene|0A9FF5A66F73464A780914A1424979CE753B3D9C|murD]-[gene|5DE602D97D903D9AE38ED05D5A5F1B3A2DC1D58E|spoVE]-[gene|D52FDB5015B10E9B26D7FED5708457890C6EA43D|murG]-[gene|5E11616A51EE2CD1B8D3E33B18483521C504AD1F|murB]-[gene|B66FC02948DABBCE64DE2629D6E33E05647B4EEF|divIB]-[gene|5CDB49209BDCA86E09D6287DFC16B278D9A71379|ylxW]-[gene|081EA0CBCDA7ECDE8D128DD091C132BB194D19EB|ylxX]-[gene|1C6CADD1808CA84CBC19D623DACF4D398EB43174|sbp]

  description: [Pubmed|1391053]

  regulation

  • expressed during vegatative growth [Pubmed|1391053]

  regulatory mechanism

  • stringent response: negative regulation, in [regulon|other_regulator:stringent response|stringent response]
  • [protein|90DCE4F286D2C151D8BDBF0E024E6BBEC94E2397|spoIIID]: repression, [Pubmed|15383836], in [regulon|protein:90DCE4F286D2C151D8BDBF0E024E6BBEC94E2397|spoIIID regulon]

  sigma factors

  • [protein|3CB24D493B3282278B7DC61CE3C793DFA815F9E2|sigE]: sigma factor, [Pubmed|8320223], in [regulon|protein:3CB24D493B3282278B7DC61CE3C793DFA815F9E2|sigE regulon]

References

Reviews

• 36411352

Original Publications

• 8436954,18063720,1391053