murB

168

UDP-N-acetylenolpyruvoylglucosamine reductase

Locus: BSU_15230

Molecular weight: 32.65 kDa

Isoelectric point: 6.09

Protein length: 303 aa

Gene length: 912 bp

Function: peptidoglycan precursor biosynthesis

Product: UDP-N-acetylenolpyruvoylglucosamine reductase

Essential: yes

E.C.: 1.1.1.158

Synonyms: murB, ylxC

Genomic Context

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List of homologs in different organisms, belongs to COG0812 (Galperin et al., 2021)

Gene

Coordinates: 1,592,663  1,593,574

Phenotypes of a mutant

• essential PubMed

The protein

Catalyzed reaction/ biological activity

• NADP+ + UDP-N-acetyl-α-D-muramate --> H⁺ + NADPH + UDP-N-acetyl-3-O-(1-carboxyvinyl)-α-D-glucosamine (according to UniProt)

Protein family

• MurB family (single member, according to UniProt)

Domains

• FAD-binding PCMH-type domain (aa 29-196) (according to UniProt)

Cofactors

• FAD (according to UniProt)

Structure

• 2GQU (PDB) (from Thermus caldophilus, 38% identity, 55% similarity) PubMed
• P18579 (AlphaFold)

Localization

• forms banded patterns along the length of the cell, this pattern formation depends on MreB PubMed

Expression and Regulation

Operons

• Genes: murG-murB-divIB-ylxW-ylxX-sbp

  Description: PubMed

  Regulation

  • expressed during vegatative growth, then switched off, and again expressed during sporulation in the mother cell (SigE) PubMed

  Regulatory mechanism

  • SpoIIID: repression, PubMed, in spoIIID regulon

  Sigma factors

  • SigM: sigma factor, (promoter within MurG) PubMed, in sigM regulon
  • SigA: sigma factor, PubMed, in sigA regulon
  • SigE: sigma factor, PubMed, in sigE regulon

• Genes: murE-mraY-murD-spoVE-murG-murB-divIB-ylxW-ylxX-sbp

  Description: PubMed

  Regulation

  • expressed during vegatative growth PubMed

  Regulatory mechanism

  • stringent response: negative regulation, in stringent response
  • SpoIIID: repression, PubMed, in spoIIID regulon

  Sigma factors

  • SigE: sigma factor, PubMed, in sigE regulon

• Genes: spoVE-murG-murB-divIB-ylxW-ylxX-sbp

  Description: PubMed

  Regulation

  • expressed during vegatative growth, then switched off, and again expressed during sporulation in the mother cell (SigE) PubMed

• Genes: murB-divIB-ylxW-ylxX-sbp

  Description: PubMed

  Regulation

  • expressed during vegatative growth, then switched off, and again expressed during sporulation in the mother cell (SigE) PubMed

Labs working on this gene/protein

• Adriano Henriques, Lisbon, Portugal homepage

References

Reviews

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Original Publications

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