mtnN

168

methylthioadenosine nucleosidase

locus: BSU_27270

Molecular weight: 25.12 kDa

pI: 4.84

Protein length: 231 aa

Gene length: 696 bp

function: methionine salvage

product: methylthioadenosine nucleosidase

essential: no

ec: 3.2.2.9

synonyms: mtnN, yrrU, mtn, mthA

List of homologs in different organisms, belongs to COG0775 (Galperin et al., 2021)

Gene

Coordinates: 2,787,130  2,787,825

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The protein

Catalyzed reaction/ biological activity

• H₂O + S-adenosyl-L-homocysteine --> adenine + S-(5-deoxy-D-ribos-5-yl)-L-homocysteine (according to UniProt)
• H₂O + S-methyl-5'-thioadenosine --> adenine + S-methyl-5-thio-D-ribose (according to UniProt)

Protein family

• PNP/UDP phosphorylase family (with [protein|3075327DBE55C4F628171AEE248CAED5870693FA|deoD], according to UniProt)

Structure

• [PDB|1JYS] (from ''Escherichia coli'', 51% identity, 70% similarity) [Pubmed|11591349]
• [AF|O32028]

Biological materials

Mutant

• MGNA-A850 (yrrU::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/850 NBRP B. subtilis, Japan]
• BKE27270 ([gene|5B21D1E73A65B13CE4DDA32ABC71EFA54E8D1D8F|mtnN]::erm  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE27270 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_CATCTATTCACATTCCCTTC,  downstream forward: _UP4_TAAAACGGGGAGAACGTTCT
• BKK27270 ([gene|5B21D1E73A65B13CE4DDA32ABC71EFA54E8D1D8F|mtnN]::kan  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK27270 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_CATCTATTCACATTCCCTTC,  downstream forward: _UP4_TAAAACGGGGAGAACGTTCT

References

• 17056751,16513748,12642660,15102328,16885442,24509311