mtlD

168

mannitol-1-phosphate 5-dehydrogenase

Locus: BSU_03990

Molecular weight: 40.31 kDa

Isoelectric point: 6.29

Protein length: 373 aa

Gene length: 1122 bp

Function: mannitol utilization

Product: mannitol-1-phosphate 5-dehydrogenase

Essential: no

E.C.: 1.1.1.17

Synonyms: mtlD, mtlB

Genomic Context

Hide small RNAsZoom:

List of homologs in different organisms, belongs to COG0246 (Galperin et al., 2021)

Gene

Coordinates: 451,618  452,739

The protein

Catalyzed reaction/ biological activity

• D-mannitol 1-phosphate + NAD⁺ --> β-D-fructose 6-phosphate + NADH (according to UniProt)

Protein family

• mannitol dehydrogenase family (with UxaB, according to UniProt)

Structure

• 3H2Z (PDB) (from Shigella flexneri 2a str. 2457t mutant, 42% identity, 59% similarity)
• P42957 (AlphaFold)

Expression and Regulation

Operons

• Genes: mtlA-mtlF-mtlD

  Description: PubMed

  Regulation

  • induced by mannitol (MtlR) PubMed

  Regulatory mechanism

  • MtlR: activation, PubMed, in mtlR regulon

  Sigma factors

  • SigA: sigma factor, PubMed, in sigA regulon

  Additional information

  • An antisense RNA is predicted for mtlD PubMed

Biological materials

Mutant

• MGNA-C019 (mtlD::erm), available at the NBRP B. subtilis, Japan
• BKE03990 (mtlD::erm  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CGCACCGAAATGTAAGGCGA,  downstream forward: _UP4_TAACCGACCACCCGTGACAC
• BKK03990 (mtlD::kan  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CGCACCGAAATGTAAGGCGA,  downstream forward: _UP4_TAACCGACCACCCGTGACAC

References

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