mtlA

mtlA
168

mannitol-specific permease of the [wiki|phosphotransferase systems|phosphotransferase system], EIICB of the [category|SW.1.2.2|PTS], [category|SW.3.4.3|Trigger enzyme], control of [protein|1CEDC98C9EDD8F3DEA0AF4B2104DA1BF86E688C2|mtlR] activity

locus
BSU_03981
Molecular weight
50.00 kDa
pI
8.814
Protein length
Gene length
function
mannitol uptake and phosphorylation, control of [protein|1CEDC98C9EDD8F3DEA0AF4B2104DA1BF86E688C2|mtlR] activity
product
mannitol-specific [category|SW.1.2.2|PTS], EIICB
essential
no
ec
2.7.1.69
synonyms
mtlA

Genomic Context

Categories containing this gene/protein

List of homologs in different organisms, belongs to COG2213 (Galperin et al., 2021)

This gene is a member of the following regulons

Gene
Coordinates
449,724  451,160
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The protein
Catalyzed reaction/ biological activity
D-mannitol + Nπ-phospho-L-histidyl-[protein] --> D-mannitol 1-phosphate + L-histidyl-[protein] (according to UniProt)
Protein family
[category|SW.1.2.2|PTS] permease, fructose/ mannitol family [Pubmed|10627040]
[wiki|Domains]
2 [wiki|PTS EIIC domain]s type-2 (aa 18-347, aa 390-478) (according to UniProt)
Structure
[PDB|1A3A] (from ''Escherichia coli k12 mutant'', 42% identity, 66% similarity)\t[Pubmed|9551558]
[AF|P42956]
Modification
phosphorylation on Ser-559 [Pubmed|17218307]
[wiki|Localization]
cell membrane (according to UniProt)
Expression and Regulation
Operons
genes
[gene|5291FEF41CA547223EDF9EBAFA38D3EE550ED3F3|mtlA]-[gene|EE4A4336E25F5DCAC89214F55CDE203AB47AA249|mtlF]-[gene|4D54898D405EA25A0C74B1F02A492143D49B7B07|mtlD]
description
[Pubmed|12897001]
regulation
induced by mannitol ([protein|1CEDC98C9EDD8F3DEA0AF4B2104DA1BF86E688C2|mtlR]) [Pubmed|20444094]
regulatory mechanism
[protein|1CEDC98C9EDD8F3DEA0AF4B2104DA1BF86E688C2|mtlR]: activation, [Pubmed|20444094], in [regulon|protein:1CEDC98C9EDD8F3DEA0AF4B2104DA1BF86E688C2|mtlR regulon]
sigma factors
[protein|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|sigA]: sigma factor, [Pubmed|22014119], in [regulon|protein:360F48D576DE950DF79C1A2677B7A35A8D8CC30C|sigA regulon]
additional information
An [wiki|ncRNA|antisense RNA] is predicted for [gene|4D54898D405EA25A0C74B1F02A492143D49B7B07|mtlD] [PubMed|20525796]
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[gene|5291FEF41CA547223EDF9EBAFA38D3EE550ED3F3|mtlA]→[gene|4D54898D405EA25A0C74B1F02A492143D49B7B07|mtlD]

2024-03-03 13:53:09

Jstuelk

39

5bae19b2d80b020443468ffbe472a91dbcc86cee

F44226BCF3EE1A7B00EEDFF583F5DD4C12739D38

Biological materials
Mutant
MGNA-C018 (mtlA::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/2016 NBRP B. subtilis, Japan]
BKE03981 ([gene|5291FEF41CA547223EDF9EBAFA38D3EE550ED3F3|mtlA]::erm  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE03981 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_CATATATAAACCCTCCCTGT,  downstream forward: _UP4_TAATCTTATAGAAAGAGAGT
BKK03981 ([gene|5291FEF41CA547223EDF9EBAFA38D3EE550ED3F3|mtlA]::kan  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK03981 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_CATATATAAACCCTCCCTGT,  downstream forward: _UP4_TAATCTTATAGAAAGAGAGT
References
Reviews
23318733
Original Publications
12897001,20444094,10627040,17218307,23279188,22014119

5291FEF41CA547223EDF9EBAFA38D3EE550ED3F3

Page visits: 3845

Time of last update: 2024-03-28 23:41:33

Author of last update: Melvin.boenninger