mrpA

168

Na⁺ transporter subunit of the Na⁺/H⁺ antiporter, multiple resistance and pH homeostasis

locus: BSU_31600

Molecular weight: 86.23 kDa

pI: 9.52

Protein length: 774 aa

Gene length: 2325 bp

function: sodium export

product: Na⁺/H⁺ antiporter subunit

essential: yes

synonyms: mrpA, yufT, shaA, ntrA

List of homologs in different organisms, belongs to COG2111 (Galperin et al., 2021)

Gene

Coordinates: 3,246,679 → 3,249,003

Phenotypes of a mutant

• essential [Pubmed|12682299]
• a ''[gene|E968DECF869CAF7E247A518494AD70B554A7A25F|mrpA]'' mutant is viable at pH7.4 and sodium concentrations below 80 mM [Pubmed|24139955]
• the ''[gene|E968DECF869CAF7E247A518494AD70B554A7A25F|mrpA]'' mutant accumulates internal sodium [Pubmed|24139955]

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The protein

Catalyzed reaction/ biological activity

• Na  transporter subunit of the Na /H  antiporter [Pubmed|21236240]

Protein family

• CPA3 antiporters (TC 2.A.63) subunit A family (single member, according to UniProt)

Structure

• [PDB|4HE8] (from ''Thermus thermophilus'', 36% identity in a 57% fragment) [Pubmed|23417064]
• [AF|Q9K2S2]

[wiki|Localization]

• inner spore membrane [Pubmed|26731423]

Expression and Regulation

Operons

• genes: [gene|E968DECF869CAF7E247A518494AD70B554A7A25F|mrpA]-[gene|AAB15E7E3E3701AA3159370B85C151994244BA5B|mrpB]-[gene|D921139B18A665E9BB177C973FCF050A4BFFF97F|mrpC]-[gene|051D9BCC0CB05E39ED5825BB9BA77B8228EA055D|mrpD]-[gene|BF233467FAF95EEEE12A0D36C543DB357D8879EA|mrpE]-[gene|5CA2EA2D60FE4696903C16698C8329A20CBEBF72|mrpF]-[gene|B28796716C7E6C924F55176453DDBF9ADD0EF755|mrpG]

  description: [Pubmed|9274030,10198001]

Biological materials

Mutant

• MGNA-B557 (yufT::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/1556 NBRP B. subtilis, Japan]

References

Reviews

• 29218041

Original Publications

• 15019740,11004162,17693497,10198001,19389778,16730649,21236240,24021651,24095649,24139955,26731423,23417064,27391676,18408029