iolA

168

methylmalonate-semialdehyde dehydrogenase (acylating)

locus: BSU_39760

Molecular weight: 53.29 kDa

pI: 5.14

Protein length: 487 aa

Gene length: 1464 bp

function: myo-inositol catabolism

product: methylmalonate-semialdehyde dehydrogenase (acylating)

essential: no

ec: 1.2.1.27

synonyms: iolA, mmsA, yxdA

List of homologs in different organisms, belongs to COG1012 (Galperin et al., 2021)

Gene

Coordinates: 4,082,920  4,084,383

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The protein

Catalyzed reaction/ biological activity

• NAD-dependent oxidation of methylmalonate semialdehyde (MMSA) to propionyl-CoA via acylation and deacylation steps
• 3-oxopropanoate + CoA + NAD⁺ --> acetyl-CoA + CO₂ + NADH (according to UniProt)
• 2-methyl-3-oxopropanoate + CoA + H₂O + NAD⁺ --> H⁺ + hydrogencarbonate + NADH + propanoyl-CoA (according to UniProt)

Protein family

• [wiki|aldehyde dehydrogenase family] (according to UniProt)

[wiki|Cofactors]

• Coenzyme A, NAD [Pubmed|22782904]

Structure

• [PDB|1T90]\t [Pubmed|22782904]
• [AF|P42412]

Paralogous protein(s)

• [protein|99F1FAF28FB4817D94E84BD5288FA33124558933|ywdH], [protein|A0BEB92D54799956A4ADE106A1388E5710141069|gabD], [protein|EF0AAAF5BAE8E1F54FCA296643F7B9E3CE257B1D|yfmT], [protein|F3341F205CB939498109D2A54DE842065C488DD5|aldX], [protein|F9CF067A2A8E3B2BACC6A51A0E87E84640349980|aldY], [protein|1ADC55F8E265AF86E29743C671CE1EA1BE7EB41E|gbsA], [protein|2042F691CB37B1BAFE2CF3906A9F7F47457C3995|putC], [protein|5B66ADB81AB141982F187B1C5E3A9346AA064DE2|ycbD], [protein|67A72A0EABCD807C25D8EAC61C251142B45C174E|dhaS], [protein|69838717DC6BB27864D88C282BF5BC7CC558BFD7|rocA]

Biological materials

Mutant

• MGNA-B770 (iolA::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/1769 NBRP B. subtilis, Japan]
• BKE39760 ([gene|762718A15E5256261D79DF60F9106AF0CE2D60C6|iolA]::erm  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE39760 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_CATTCTTATTGCCTCCTTCA,  downstream forward: _UP4_TAAACGACGAACAGCCGAAC
• BKK39760 ([gene|762718A15E5256261D79DF60F9106AF0CE2D60C6|iolA]::kan  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK39760 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_CATTCTTATTGCCTCCTTCA,  downstream forward: _UP4_TAAACGACGAACAGCCGAAC

labs

• [wiki|Yasutaro Fujita], University of Fukuyama, Japan
• [[Ken-ichi Yoshida]], Kobe University, Japan

References

• 9226270,11566986,10666464,18310071,9887260,9887260,18310071,16332250,22900538