minJ

minJ
168

topological determinant of [wiki|cell division], part of the Min system (with Z ring placement)

locus
BSU_35220
Molecular weight
43.51 kDa
pI
9.06
Protein length
Gene length
function
[wiki|cell division] site selection
product
topological determinant of [wiki|cell division]
essential
no
synonyms
minJ, yvjD, swrAB

Genomic Context

Categories containing this gene/protein

List of homologs in different organisms, belongs to COG0265 (Galperin et al., 2021)

This gene is a member of the following regulons

Gene
Coordinates
3,620,346 → 3,621,539
Phenotypes of a mutant
formation of minicells [pubmed|33849976]
increased cell length [pubmed|33849976,34846166]]
inactivation of [gene|C3482F13AE7B7462D9A4C91C8B461B7987A2277D|minJ] reduces sporulation efficiency to 36% that of wild type cells; delayed entry into sporulation and aberrant forespores [Pubmed|26735940]
defective in [category|SW.4.1.4|Swarming] motility [pubmed|35638827]
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The protein
Catalyzed reaction/ biological activity
The Min system prevents minicell formation adjacent to recently completed division sites by promoting the disassembly of the cytokinetic ring, thereby ensuring that cell division occurs only once per cell cycle  [Pubmed|20352045]
required for oriC placement during spore development [Pubmed|27059541]
Protein family
MinJ family (single member, according to UniProt)
[wiki|Domains]
7 transmembrane helices (aa 15- 269) (according to UniProt)
[wiki|PDZ domain] (aa 295 - 361) (according to UniProt)
Structure
[AF|O34375]
[wiki|Localization]
forms rings at the division septum [Pubmed|22108385]
cell membrane [Pubmed|23264578]
Additional information
about 580 molecules per cell [pubmed|33849976]
Expression and Regulation
Operons
genes
[gene|0CEE58D799AF41634D161DBDF3D67EEFFF1C861E|swrAA/2]-[gene|C3482F13AE7B7462D9A4C91C8B461B7987A2277D|minJ]
description
[Pubmed|16030230]
regulatory mechanism
[protein|D343D2096664A972026D911E7A17A35C7B1CD1C9|degU]: activation, [protein|D343D2096664A972026D911E7A17A35C7B1CD1C9|degU]-P, ([protein|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|sigA] promoter) [Pubmed|18567663], in [regulon|protein:D343D2096664A972026D911E7A17A35C7B1CD1C9|degU regulon]
sigma factors
[protein|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|sigA]: sigma factor, [Pubmed|18567663], in [regulon|protein:360F48D576DE950DF79C1A2677B7A35A8D8CC30C|sigA regulon]
[protein|7024E4162A6D827069F882FDEACA696EBC05DD40|sigD]: sigma factor, [Pubmed|18567663], in [regulon|protein:7024E4162A6D827069F882FDEACA696EBC05DD40|sigD regulon]
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[gene|0CEE58D799AF41634D161DBDF3D67EEFFF1C861E|swrAA/2]→[gene|C3482F13AE7B7462D9A4C91C8B461B7987A2277D|minJ]

2024-02-29 22:40:13

ghost

82

af433e194a5c1071d0c7300669e650f4e7ec7979

1B5159BD9F121BFDFA8F3B4B3F78047C7301257A

genes
[gene|C3482F13AE7B7462D9A4C91C8B461B7987A2277D|minJ]
description
[pubmed|18976281]
Open in new tab

[gene|C3482F13AE7B7462D9A4C91C8B461B7987A2277D|minJ]

2024-03-09 15:19:40

Jstuelk

63

738a69fc0f835236ae1fe61af58643496efd67e0

A14B0EBE23F828695FBFE67DC36E860926BD8B61

Biological materials
Mutant
MGNA-B648 (minJ::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/1647 NBRP B. subtilis, Japan]
BKE35220 (Δ[gene|C3482F13AE7B7462D9A4C91C8B461B7987A2277D|minJ]::erm  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE35220 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_CACTATCTCTCACCGCCTCA,  downstream forward: _UP4_TAAAAGGCAGCCCGGCACCG
BKK35220 (Δ[gene|C3482F13AE7B7462D9A4C91C8B461B7987A2277D|minJ]::kan  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK35220 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_CACTATCTCTCACCGCCTCA,  downstream forward: _UP4_TAAAAGGCAGCCCGGCACCG
two-hybrid system
''B. pertussis'' adenylate cyclase-based bacterial two hybrid system ([wiki|BACTH]), available in [wiki|Jörg Stülke]'s lab
labs
[wiki|Daniel Kearns], Indiana University, Bloomington, USA, [http://www.bio.indiana.edu/facultyresearch/faculty/Kearns.html homepage]
[wiki|Marc Bramkamp], University of Cologne, Cologne, Germany [http://www.kraemerlab.uni-koeln.de/celldivision.php homepage]
References
Reviews
19884039,24367361,28500523,35638784
Original Publications
19019154,16030230,18976281,20352045,18567663,22108385,22457634,23264578,26735940,27059541,28674273,29403445,33849976,34846166,35205323,35638827

C3482F13AE7B7462D9A4C91C8B461B7987A2277D

Page visits: 5133

Time of last update: 2024-03-28 10:59:21

Author of last update: Jstuelk