minD
168
[wiki|cell division] inhibitor (septum placement), part of the Min system (with Z ring placement), required for the disassembly of polar [protein|41872E2EF00C79918DD077F2EF78F37E24FEB110|Z rings] after [wiki|cell division]
locus
BSU_27990
Molecular weight
29.26 kDa
pI
4.98
function
septum placement
product
[wiki|cell division] inhibitor
essential
no
synonyms
minD, divIVB1
Outlinks
Genomic Context
Categories containing this gene/protein
List of homologs in different organisms, belongs to COG2894 (Galperin et al., 2021)
This gene is a member of the following regulons
Gene
Coordinates
2,857,776 2,858,582
Phenotypes of a mutant
produces minicells [pubmed|33849976,32184253]
increased cell length [pubmed|33849976]
a [gene|1986DB3078FBA16D6ACCD70EEAC95C46564D0958|whiA] [gene|8C94C9598A823A8405B3E1FA0124E21D90845B8E|minC]-[gene|37DAD42A391E2FC506225EAF91B8F21629A401DF|minD] mutant grows poorly, and the cells are filamentous [Pubmed|24097947]
severe growth defect of a [gene|37DAD42A391E2FC506225EAF91B8F21629A401DF|minD] [gene|559DEDC9887B811EF80994526256ADC48BA51CE3|noc] double mutant at increased temperature (37°C or above) due to a maturation defect of the Z-ring for [wiki|cell division], this can be suppressed by any mutation affecting the [protein|297F53DAD3351E0C55108DD2C93B78FFB174438C|clpX]-[protein|CB06A70DE7462CEB7AF5D8C28943C878DD56DE1A|clpP]-[protein|87124945A1CBF7990856FBEB9EAD25096DAC0868|spxH] proteolytic system due to [protein|2C6386E9A63F410558D168798D077DF91590F454|spx]-mediated increased expression of [protein|EAD44AA7D72510B70308FD0C9F159DBEDAEBBB74|zapA] [pubmed|34181483]
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The protein
Catalyzed reaction/ biological activity
The Min system prevents minicell formation adjacent to recently completed division sites by promoting the disassembly of the cytokinetic ring, thereby ensuring that cell division occurs only once per cell cycle [Pubmed|20352045]
required for oriC placement during spore development [Pubmed|27059541]
Protein family
ParA family (with [protein|D6D34E68FB3BAAACB533F98290FD363A5B00B2C6|flhG] and [protein|5B3DB5A796ACA48390278E60478DFF13D0074A8D|parA], according to UniProt)
Structure
[PDB|4V03] (from ''Aquifex aeolicus'', 51% identity) [Pubmed|25500731]
[AF|Q01464]
[wiki|Localization]
polar/septal at the cell membrane [Pubmed|20566861]
membrane binding/polar localization depends on the proton motive force [Pubmed|20566861]
Additional information
about 3,500 molecules per cell [pubmed|33849976]
Expression and Regulation
Operons
genes
[gene|A4C8719E06F774A6EB4D79757CC79CF89E453A54|mreB]-[gene|6A14D0C7FD94A838BAB3B74AA100EA678F1E4E34|mreC]-[gene|231C30963AFB863424C087CB722446D319976D65|mreD]-[gene|8C94C9598A823A8405B3E1FA0124E21D90845B8E|minC]-[gene|37DAD42A391E2FC506225EAF91B8F21629A401DF|minD]
description
[Pubmed|8459776]
regulation
constitutively expressed [Pubmed|23701187]
regulatory mechanism
[protein|08CFA2C72931A75532D4289BC1D18A826DE9F9CA|comK]: activation, [Pubmed|26091431], in [regulon|protein:08CFA2C72931A75532D4289BC1D18A826DE9F9CA|comK regulon]
sigma factors
[protein|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|sigA]: sigma factor, promoter p1, upstream of [protein|49D996C9AED44A820A214C3DF60AB1B6E1508DEA|maf] [Pubmed|26091431], in [regulon|protein:360F48D576DE950DF79C1A2677B7A35A8D8CC30C|sigA regulon]
[protein|081DF3EE9FA56209D648C7677188C61CE3AA8E41|sigM]: sigma factor, promoter p2, within [protein|49D996C9AED44A820A214C3DF60AB1B6E1508DEA|maf] [Pubmed|18179421], in [regulon|protein:081DF3EE9FA56209D648C7677188C61CE3AA8E41|sigM regulon]
[protein|DC3449D5F195E5C2E9E14FEC95396C8F1FDF73B4|sigH]: sigma factor, promoter p4, upstream of [protein|8C94C9598A823A8405B3E1FA0124E21D90845B8E|minC] [Pubmed|8459776], in [regulon|protein:DC3449D5F195E5C2E9E14FEC95396C8F1FDF73B4|sigH regulon]
genes
[gene|8C94C9598A823A8405B3E1FA0124E21D90845B8E|minC]-[gene|37DAD42A391E2FC506225EAF91B8F21629A401DF|minD]
description
[Pubmed|8459776]
regulation
constitutively expressed [Pubmed|23701187]
genes
[gene|49D996C9AED44A820A214C3DF60AB1B6E1508DEA|maf]-[gene|2BD11D71AA6D9EFAD8A209C8813CC8B059180C84|radC]-[gene|A4C8719E06F774A6EB4D79757CC79CF89E453A54|mreB]-[gene|6A14D0C7FD94A838BAB3B74AA100EA678F1E4E34|mreC]-[gene|231C30963AFB863424C087CB722446D319976D65|mreD]-[gene|8C94C9598A823A8405B3E1FA0124E21D90845B8E|minC]-[gene|37DAD42A391E2FC506225EAF91B8F21629A401DF|minD]
description
[Pubmed|22383849]
regulation
constitutively expressed [Pubmed|23701187]
regulatory mechanism
[protein|08CFA2C72931A75532D4289BC1D18A826DE9F9CA|comK]: activation, [Pubmed|11948146,11918817,21564336], in [regulon|protein:08CFA2C72931A75532D4289BC1D18A826DE9F9CA|comK regulon]
sigma factors
[protein|081DF3EE9FA56209D648C7677188C61CE3AA8E41|sigM]: sigma factor, promoter p2 within [protein|49D996C9AED44A820A214C3DF60AB1B6E1508DEA|maf] [Pubmed|18179421], in [regulon|protein:081DF3EE9FA56209D648C7677188C61CE3AA8E41|sigM regulon]
[protein|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|sigA]: sigma factor, promoter p1, upstream of [protein|49D996C9AED44A820A214C3DF60AB1B6E1508DEA|maf] [Pubmed|26091431], in [regulon|protein:360F48D576DE950DF79C1A2677B7A35A8D8CC30C|sigA regulon]
Biological materials
Mutant
BKE27990 ([gene|37DAD42A391E2FC506225EAF91B8F21629A401DF|minD]::erm trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE27990 BGSC], [Pubmed|28189581], upstream reverse: _UP1_AGTTATTACGATAGCCTCAC, downstream forward: _UP4_TAATGTGATAGAATCAAAGA
BKK27990 ([gene|37DAD42A391E2FC506225EAF91B8F21629A401DF|minD]::kan trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK27990 BGSC], [Pubmed|28189581], upstream reverse: _UP1_AGTTATTACGATAGCCTCAC, downstream forward: _UP4_TAATGTGATAGAATCAAAGA
two-hybrid system
''B. pertussis'' adenylate cyclase-based bacterial two hybrid system ([wiki|BACTH]), available in [wiki|Jörg Stülke]'s lab
References
Reviews
Original Publications
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Time of last update: 2024-04-19 20:14:17
Author of last update: Jstuelk