menH

168

menaquinone biosynthesis methyltransferase

locus: BSU_22750

Molecular weight: 26.43 kDa

pI: 8.17

Protein length: 233 aa

Gene length: 702 bp

function: biosynthesis of menaquinone

product: menaquinone biosynthesis methyltransferase

essential: no

ec: 2.1.1.163

synonyms: menH, gerCB, menG

List of homologs in different organisms, belongs to COG2226 (Galperin et al., 2021)

Gene

Coordinates: 2,382,907 → 2,383,608

Phenotypes of a mutant

• essential [http://www.ncbi.nlm.nih.gov/pubmed/?term=12682299 according to Kobayashi et al.]; however, inactivation reported by [http://www.ncbi.nlm.nih.gov/pubmed/?term=26735940 Meeske et al.] and [http://www.ncbi.nlm.nih.gov/pubmed/?term=20511502 Block et al.]
• inactivation of ''[gene|DDD40AEBBCBD3C19661A72B85912E266DBF267B3|menH]'' reduces sporulation efficiency to 12.7% that of wild type cells [Pubmed|26735940]
• defective in biofilm formation [pubmed|31420537]

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The protein

Catalyzed reaction/ biological activity

• 2-demethylmenaquinol + S-adenosyl-L-methionine --> menaquinol + H⁺ + S-adenosyl-L-homocysteine (according to UniProt)

Protein family

• [wiki|Methyltransferase superfamily] (according to UniProt)
• [wiki|class I-like SAM-binding methyltransferase superfamily] (according to UniProt)

[wiki|Domains]

• [wiki|AB hydrolase-1 domain] (aa 26-259) (according to InterPro)

Structure

• [PDB|1OBW] (from ''Saccharomyces cerevisiae'', 31% identity) [Pubmed|9201917]
• [AF|P31113]

Expression and Regulation

Operons

• genes: [gene|C45A5935559F0DAAD70F808725AF730BC18A2B0A|hepS]-[gene|DDD40AEBBCBD3C19661A72B85912E266DBF267B3|menH]-[gene|3CAEA54E80A0B56EE120B881C8CDBE055AF6BDC1|hepT]-[gene|B498ED03F19B7A73878D5C947C28C2A87CAB934D|ndk]

  description: [pubmed|22383849]

additional information

• highly expressed at high iron concentrations [pubmed|31420537]

Biological materials

Mutant

• BKE22750 (Δ[gene|DDD40AEBBCBD3C19661A72B85912E266DBF267B3|menH]::erm  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE22750 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_TTCTTTTGAGTCCTGCATAA,  downstream forward: _UP4_CTGTTTGAAGAGGCGGGCCT
• BKK22750 (Δ[gene|DDD40AEBBCBD3C19661A72B85912E266DBF267B3|menH]::kan  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK22750 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_TTCTTTTGAGTCCTGCATAA,  downstream forward: _UP4_CTGTTTGAAGAGGCGGGCCT

References

• 9201917,26735940,20511502,31420537