menA

168

probable 1,4-dihydroxy-2-naphthoate octaprenyltransferase

locus: BSU_38490

Molecular weight: 33.68 kDa

pI: 9.32

Protein length: 311 aa

Gene length: 936 bp

function: biosynthesis of menaquinone

product: probable 1,4-dihydroxy-2-naphthoate octaprenyltransferase

essential: yes

ec: 2.5.1.74

synonyms: menA, ywaB, ipa-6d

List of homologs in different organisms, belongs to COG1575 (Galperin et al., 2021)

Gene

Coordinates: 3,950,726  3,951,661

Phenotypes of a mutant

• essential [Pubmed|12682299]

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The protein

Catalyzed reaction/ biological activity

• 1,4-dihydroxy-2-naphthoate + all-trans-polyprenyl diphosphate + H⁺ --> 2-demethylmenaquinol + CO₂ + diphosphate (according to UniProt)

Protein family

• MenA family (single member, according to UniProt)

Structure

• [PDB|3BYV] (from ''Toxoplasma gondii'', 30% identity in a 25% fragment) [Pubmed|19197235]
• [AF|P39582]

[wiki|Localization]

• cell membrane (according to UniProt)

Biological materials

Mutant

• MGNA-B217 (ywaB::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/1216 NBRP B. subtilis, Japan]

References

• 32527527
• 9353933,19197235,29794222,35643569