mcsA

168

modulator of [protein|908DB17A39D518E84977250C55825E77FA02E391|ctsR]-dependent repression, [protein|CA0939064E70A97434FA450A69E779FD89880613|mcsA] activates kinase activity of [protein|7B1B664A1AE1F641E8E7D9E2894D5C8FFFA92948|mcsB]

locus: BSU_00840

Molecular weight: 20.88 kDa

pI: 6.62

Protein length: 185 aa

Gene length: 558 bp

function: control of [protein|908DB17A39D518E84977250C55825E77FA02E391|ctsR] activity

product: activator of [protein|7B1B664A1AE1F641E8E7D9E2894D5C8FFFA92948|mcsB] kinase activity

essential: no

synonyms: mcsA, yacH

List of homologs in different organisms, belongs to COG3880 (Galperin et al., 2021)

Gene

Coordinates: 101,927 → 102,484

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The protein

[wiki|Domains]

• [wiki|UVR domain] (aa 139-174) (according to UniProt)

Structure

• [AF|P37569]

Modification

• phosphorylated on Arg-169 [Pubmed|22517742]

Biological materials

Mutant

• MGNA-B929 (yacH::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/1928 NBRP B. subtilis, Japan]
• ''mcsA::aphA3'' available from the Gerth lab
• BKE00840 (Δ[gene|CA0939064E70A97434FA450A69E779FD89880613|mcsA]::erm  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE00840 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_CAATCTTTTCACCCGCTTAT,  downstream forward: _UP4_GATAGTGAGGAGGAACAGGA
• BKK00840 (Δ[gene|CA0939064E70A97434FA450A69E779FD89880613|mcsA]::kan  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK00840 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_CAATCTTTTCACCCGCTTAT,  downstream forward: _UP4_GATAGTGAGGAGGAACAGGA

Expression vectors

• for expression, purification in E. coli with N-terminal His-tag, pRSETA available in Gerth lab

Antibody

• available in Gerth lab

References

• 12923084,8793870,11179229,9987115,16497325,19226326,9987115,11544224,22517742