mcpA

mcpA
168

membrane-bound chemotaxis receptor, methyl-accepting chemotaxis protein, acts also as pH sensor, responds to decreasing pH as an attractant signal

locus
BSU_31240
Molecular weight
72.22 kDa
pI
4.99
Protein length
Gene length
function
control of chemotaxis, sensing of acidic enviromnents
product
methyl-accepting chemotaxis protein
essential
no
synonyms
mcpA

Genomic Context

Categories containing this gene/protein

List of homologs in different organisms, belongs to COG0840 (Galperin et al., 2021)

This gene is a member of the following regulons

Gene
Coordinates
3,206,169 → 3,208,154
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The protein
Catalyzed reaction/ biological activity
responsible for repellent response towards phenol and other aromatics (via the signaling domain) [pubmed|35007157]
[wiki|Domains]
[wiki|Cache domain] (aa 152-228) (according to UniProt)
[wiki|HAMP domain] (aa 303-355) (according to UniProt)
[wiki|Methyl-accepting transducer domain] (aa 374-610) (according to UniProt)
Structure
[PDB|6S1K] (from E. coli, corresponds to aa 291 ... 555, 31% identity) [pubmed|31925330]
[AF|P39214]
Modification
deamination of Gln-586, Gln-593, and Gln-594 (by [protein|BE9373BEB682E5F8B0938AAA4ED9B723B357ABAE|cheD]) [Pubmed|22931217]
Paralogous protein(s)
[protein|E4E1020B799A1B403BDB4847B91B02D64D161AF3|mcpB], [protein|4CD44AD8FE68516C84889EAA2137828E06B8A30B|tlpB], [protein|1DE26CDEE952142C1303C822F0E2A63AE09F721B|tlpA], [protein|8333CD46F704F03A22482CAA98DEFCC945362A10|mcpC]
[wiki|Localization]
cell membrane [Pubmed|18763711,21515776]
Expression and Regulation
Operons
genes
[gene|1DE26CDEE952142C1303C822F0E2A63AE09F721B|tlpA]-[gene|F02E3080214A327E131ECDE0DBC52C57EFDFE2CE|mcpA]
description
[Pubmed|8188684]
regulatory mechanism
[protein|E5110D9C36E8C55AFD1419987B14A53232165F20|abrB]: repression, [Pubmed|20817675], in [regulon|protein:E5110D9C36E8C55AFD1419987B14A53232165F20|abrB regulon]
sigma factors
[protein|7024E4162A6D827069F882FDEACA696EBC05DD40|sigD]: sigma factor, [Pubmed|8188684], in [regulon|protein:7024E4162A6D827069F882FDEACA696EBC05DD40|sigD regulon]
additional information
in minimal medium, McpA is present with 15,900 +/- 3,000 molecules per cell [PubMed|21515776]
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[gene|1DE26CDEE952142C1303C822F0E2A63AE09F721B|tlpA]→[gene|F02E3080214A327E131ECDE0DBC52C57EFDFE2CE|mcpA]

2024-01-20 05:11:05

ghost

57

d93fadc070f0651f242c5fa096ab3f61c3c656b2

978EA1700C85B753C575F28E2A583FE04FD4E8F7

Biological materials
Mutant
BKE31240 (Δ[gene|F02E3080214A327E131ECDE0DBC52C57EFDFE2CE|mcpA]::erm  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE31240 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_CATCATTCATTTCTCCTTTT,  downstream forward: _UP4_TAATAAGCCTTAACACCCAA
BKK31240 (Δ[gene|F02E3080214A327E131ECDE0DBC52C57EFDFE2CE|mcpA]::kan  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK31240 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_CATCATTCATTTCTCCTTTT,  downstream forward: _UP4_TAATAAGCCTTAACACCCAA
References
Reviews
31792011,35323015
Original Publications
8251536,6137212,2105313,8188684,2505839,18763711,21515776,22931217,31685537,31925330,35007157

F02E3080214A327E131ECDE0DBC52C57EFDFE2CE

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Time of last update: 2024-03-18 23:20:02

Author of last update: Jstuelk