maf

maf
168

nucleotide pyrophosphatase

locus
BSU_28050
Molecular weight
21.15 kDa
pI
5.71
Protein length
Gene length
function
unknown
product
nucleotide pyrophosphatase
essential
no
synonyms
maf

Genomic Context

Categories containing this gene/protein

List of homologs in different organisms, belongs to COG0424 (Galperin et al., 2021)

This gene is a member of the following regulons

Gene
Coordinates
2,862,572  2,863,141
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The protein
Catalyzed reaction/ biological activity
nucleotide pyrophosphatase [Pubmed|24210219]
dTTP + H2O --> diphosphate + dTMP + H+ (according to UniPort)
H2O + UTP --> diphosphate + H+ + UMP (according to UniPort)
CTP + H2O --> CMP + diphosphate + H+ (according to UniPort)
H2O + ψ-UTP --> diphosphate + H+ + ψ-UMP (according to UniPort)
5-methyl-CTP + H2O --> 5-methyl-CMP + diphosphate + H+ (according to UniPort)
5-methyl-UTP + H2O --> 5-methyl-UMP + diphosphate + H+ (according to UniPort)
Protein family
maf family (single member, according to UniProt)
[wiki|Cofactors]
binds phosphorylated compounds (UTP, IMP, IDP, GDP and others) in ''in vitro'' experiments
Structure
[PDB|1EX2] [Pubmed|10841541]
[AF|Q02169]
[wiki|Localization]
cytosolic protein [Pubmed|24164455]
Expression and Regulation
Operons
genes
[gene|49D996C9AED44A820A214C3DF60AB1B6E1508DEA|maf]-[gene|2BD11D71AA6D9EFAD8A209C8813CC8B059180C84|radC]-[gene|A4C8719E06F774A6EB4D79757CC79CF89E453A54|mreB]-[gene|6A14D0C7FD94A838BAB3B74AA100EA678F1E4E34|mreC]-[gene|231C30963AFB863424C087CB722446D319976D65|mreD]-[gene|8C94C9598A823A8405B3E1FA0124E21D90845B8E|minC]-[gene|37DAD42A391E2FC506225EAF91B8F21629A401DF|minD]
description
[Pubmed|22383849]
regulation
constitutively expressed [Pubmed|23701187]
regulatory mechanism
[protein|08CFA2C72931A75532D4289BC1D18A826DE9F9CA|comK]: activation, [Pubmed|11948146,11918817,21564336], in [regulon|protein:08CFA2C72931A75532D4289BC1D18A826DE9F9CA|comK regulon]
sigma factors
[protein|081DF3EE9FA56209D648C7677188C61CE3AA8E41|sigM]: sigma factor, promoter p2 within [protein|49D996C9AED44A820A214C3DF60AB1B6E1508DEA|maf] [Pubmed|18179421], in [regulon|protein:081DF3EE9FA56209D648C7677188C61CE3AA8E41|sigM regulon]
[protein|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|sigA]: sigma factor, promoter p1, upstream of [protein|49D996C9AED44A820A214C3DF60AB1B6E1508DEA|maf] [Pubmed|26091431], in [regulon|protein:360F48D576DE950DF79C1A2677B7A35A8D8CC30C|sigA regulon]
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[gene|49D996C9AED44A820A214C3DF60AB1B6E1508DEA|maf]→[gene|37DAD42A391E2FC506225EAF91B8F21629A401DF|minD]

2024-02-28 09:37:50

ghost

87

5cb36570cb9eb74ac55107ce2bd7f07e7d2d5c0b

EE31E7A5D1B0C8BDC56B004AA68A1C721D4DA9CC

Biological materials
Mutant
BKE28050 ([gene|49D996C9AED44A820A214C3DF60AB1B6E1508DEA|maf]::erm  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE28050 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_CATGTTCGTCATCCTTTCAG,  downstream forward: _UP4_TGACGGTTCTATAAACGGGA
References
Original Publications
10841541,8387996,11948146,24164455,21926231,24210219,22383849,26091431

49D996C9AED44A820A214C3DF60AB1B6E1508DEA

Page visits: 2215

Time of last update: 2024-03-15 05:14:07

Author of last update: Jstuelk