lytG

168

peptidoglycan N-acetylglucosaminidase

locus: BSU_31120

Molecular weight: 31.75 kDa

pI: 8.28

Protein length: 282 aa

Gene length: 849 bp

function: cell wall turnover

product: peptidoglycan N-acetylglucosaminidase

essential: no

synonyms: lytG, yubE

List of homologs in different organisms, belongs to COG1705 (Galperin et al., 2021)

Gene

Coordinates: 3,190,834 → 3,191,682

The protein

Catalyzed reaction/ biological activity

• hydrolysis of the glycosidic bond between N-acetyl-β-d-glucosamine residues and adjacent monosaccharides [Pubmed|18266855]

Protein family

• glycosyl hydrolase 73 family (with [protein|29219315D31BA4ADE41687EFF17FE41D6C23D157|lytD], according to UniProt)

[wiki|Domains]

• GW domain (aa 203-281) (according to UniProt)

Structure

• [AF|O32083]

[wiki|Localization]

• secreted (according to Swiss-Prot)

Expression and Regulation

Operons

• genes: [gene|AF0BC3D534BC2CCCAAB53B286CF4286E58A8A338|lytG]-[gene|E1549CE5B1648EBD1E23890D97F0B545A9EC10F6|yubF]

  description: [Pubmed|22383849]

Biological materials

Mutant

• MGNA-A027 (yubE::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/27 NBRP B. subtilis, Japan]
• BKE31120 (Δ[gene|AF0BC3D534BC2CCCAAB53B286CF4286E58A8A338|lytG]::erm  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE31120 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_CATGAAAACCTTCCTTCGAA,  downstream forward: _UP4_TAAGCCCTTTTTACTGGTAT
• BKK31120 (Δ[gene|AF0BC3D534BC2CCCAAB53B286CF4286E58A8A338|lytG]::kan  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK31120 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_CATGAAAACCTTCCTTCGAA,  downstream forward: _UP4_TAAGCCCTTTTTACTGGTAT

References

Reviews

• 18266855

Original Publications

• 12525152,17427287,28448118