lipC

lipC
168

spore coat protein, phospholipase implicated in spore germination

locus
BSU_04110
Molecular weight
23.46 kDa
pI
5.84
Protein length
Gene length
function
spore germination
product
spore coat phospholipase B
essential
no
synonyms
lipC, ycsK

Genomic Context

Categories containing this gene/protein

List of homologs in different organisms, belongs to COG2755 (Galperin et al., 2021)

This gene is a member of the following regulons

Gene
Coordinates
462,431  463,072
Phenotypes of a mutant
defective in L-alanine-stimulated germination [Pubmed|20057119]
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The protein
Catalyzed reaction/ biological activity
cleaves the fatty acids at the sn-1 and sn-2 positions of phospholipids [Pubmed|20057119]
Protein family
[wiki|'GDSL' lipolytic enzyme family] (according to UniProt)
Structure
[PDB|4PPY] (from Bacteroides fragilis, corresponds to aa 41 ... 210, 25.4% identity)
[AF|P42969]
[wiki|Localization]
spore coat (basement), localization depends on [protein|A25C1530DA7BB007A288E525404E9F775E219FE8|spoIVA] [Pubmed|22171814,17220230,20057119]
Expression and Regulation
Operons
genes
[gene|05910B4500420D4DB75DAD3A7A2358C360FF2154|lipC]
description
(according to [http://dbtbs.hgc.jp/COG/prom/ycsFGI-kipIAR-ycsK.html DBTBS]) null
regulation
expressed four hours after onset of [wiki|sporulation ]([protein|19228BAD44E6DA3DE908DC390FDF628C18D94E65|gerE]) [Pubmed|17220230]
regulatory mechanism
[protein|19228BAD44E6DA3DE908DC390FDF628C18D94E65|gerE]: activation, [Pubmed|17220230], in [regulon|protein:19228BAD44E6DA3DE908DC390FDF628C18D94E65|gerE regulon]
sigma factors
[protein|24F7FD5C7C3A68BB2760ABB8CBD8FBD65E5FF7D4|sigK]: sigma factor, [Pubmed|15699190,17220230], in [regulon|protein:24F7FD5C7C3A68BB2760ABB8CBD8FBD65E5FF7D4|sigK regulon]
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[gene|05910B4500420D4DB75DAD3A7A2358C360FF2154|lipC]

2024-01-28 12:05:10

Jstuelk

30

85f41d2e5f0ac35fd960f61b483f107564af1cfa

0B6EBB4D565DC56F1668FB2F86A2A93226F88EEA

genes
[gene|77F758CE9A59AC41F283CB1F5099097386133799|pxpA]-[gene|9AD419E3CB86C54A0F11E96C82E80A357C6B23D2|ycsG]-[gene|312E2A4ADFBCB9DD9AD9EEEC9FE53F3932AF5DBA|ycsI]-[gene|5A5E17D39296FECC1C03B1A7791AB1065BB4E4DF|pxpB]-[gene|3BA8F1F71B1D65EB30867ABD27867F925DAD2D7B|pxpC]-[gene|7DA9A79876C546B78B716A64706A3A3716018C2E|kipR]-[gene|05910B4500420D4DB75DAD3A7A2358C360FF2154|lipC]
description
[Pubmed|9334321]
regulation
induced in the presence of 5-oxoproline [pubmed|28830929]
regulatory mechanism
[protein|7DA9A79876C546B78B716A64706A3A3716018C2E|kipR]: repression, [Pubmed|9334321], in [regulon|protein:7DA9A79876C546B78B716A64706A3A3716018C2E|kipR regulon]
[protein|857EF1AC54DEA0690C01B7F8CEFFD16312C58F20|tnrA]: activation, [Pubmed|9334321], in [regulon|protein:857EF1AC54DEA0690C01B7F8CEFFD16312C58F20|tnrA regulon]
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[gene|77F758CE9A59AC41F283CB1F5099097386133799|pxpA]→[gene|05910B4500420D4DB75DAD3A7A2358C360FF2154|lipC]

2024-01-30 01:23:31

Jstuelk

53

2e345e8dee0849e85fe094a123ef62dc61c60b82

B227AB4ADF5839F5D85D0A01462013A7CDA6A81D

Biological materials
Mutant
MGNA-C024 (ycsK::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/2022 NBRP B. subtilis, Japan]
BKE04110 ([gene|05910B4500420D4DB75DAD3A7A2358C360FF2154|lipC]::erm  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE04110 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_CATGTTCTTTCCTCCACTCT,  downstream forward: _UP4_TAGAAAACGCGCCTCATGAC
BKK04110 ([gene|05910B4500420D4DB75DAD3A7A2358C360FF2154|lipC]::kan  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK04110 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_CATGTTCTTTCCTCCACTCT,  downstream forward: _UP4_TAGAAAACGCGCCTCATGAC
References
Reviews
23202530,27227299,35638784
Original Publications
17220230,9334321,20057119,22171814,25755103

05910B4500420D4DB75DAD3A7A2358C360FF2154

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Time of last update: 2024-03-19 10:20:03

Author of last update: Jstuelk