levB

168

endolevanase

Locus: BSU_34460

Molecular weight: 58.81 kDa

Isoelectric point: 4.99

Protein length: 516 aa

Gene length: 1551 bp

Function: levan degradation

Product: endolevanase

Essential: no

E.C.: 3.2.1.64

Synonyms: levB, yveB

Genomic Context

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Categories containing this gene/protein

Metabolism, Carbon metabolism, Utilization of specific carbon sources, Utilization of other polymeric carbohydrates

Groups of genes, Membrane proteins

List of homologs in different organisms, belongs to COG1621 (Galperin et al., 2021)

Gene

Coordinates: 3,537,507  3,539,057

Phenotypes of a mutant

defective in tomato root colonization in the presence of sucrose PubMed

The protein

Catalyzed reaction/ biological activity

Hydrolysis of (2->6)-beta-D-fructofuranan, to remove successive disaccharide residues as levanbiose, i.e. 6-(beta-D-fructofuranosyl)-D-fructose, from the end of the chain (according to UniProt)

Protein family

glycosyl hydrolase 32 family (with SacC and SacA, according to UniProt)

Structure

4FFF (PDB) (from Arthrobacter ureafaciens, 42% identity) PubMed
O07003 (AlphaFold)

Localization

cell membrane (according to UniProt)

Expression and Regulation

Operons

Genes: sacB-levB

Description: PubMed

Regulation

the RNA switch RNA is degraded by RNase Y PubMed

Regulatory mechanism

DegU: activation, DegU-P, PubMed, in degU regulon
SacY: antitermination, /antitermination via binding to a RNA switch, in sacY regulon

Sigma factors

SigA: sigma factor, PubMed, in sigA regulon

Biological materials

Mutant

MGNA-B614 (yveB::erm), available at the NBRP B. subtilis, Japan
BKE34460 (levB::erm  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CATATGGGATTCACCTTTAT,  downstream forward: _UP4_TAAAAACAGGGGCGGCGCAG
BKK34460 (levB::kan  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CATATGGGATTCACCTTTAT,  downstream forward: _UP4_TAAAAACAGGGGCGGCGCAG

References

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