ldcB

168

LD-carboxypeptidase, releases D-Ala from the cell wall

Locus: BSU_19620

Molecular weight: 30.70 kDa

Isoelectric point: 5.84

Protein length: 273 aa

Gene length: 822 bp

Function: cell wall synthesis

Product: LD-carboxypeptidase

Essential: no

E.C.: null

Synonyms: ldcB, yodJ

Genomic Context

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Categories containing this gene/protein

Cellular processes, Cell envelope and cell division, Cell wall synthesis, Biosynthesis of peptidoglycan

Metabolism, Additional metabolic pathways, Biosynthesis of cell wall components, Biosynthesis of peptidoglycan

List of homologs in different organisms, belongs to COG1876 (Galperin et al., 2021)

Gene

Coordinates: 2,134,566  2,135,387

The protein

Catalyzed reaction/ biological activity

trimming of cell wall peptides (tetrapeptides to tripeptides) wit concomitant release of D-ala PubMed

Protein family

peptidase M15B family (single member, according to UniProt)

Cofactors

Zn²⁺ PubMed

Structure

4OX3 (PDB) PubMed
O34866 (AlphaFold)

Localization

extracellular (signal peptide) PubMed

Expression and Regulation

Operons

Genes: deoD-ldcB

Description: PubMed

Biological materials

Mutant

MGNA-B439 (yodJ::erm), available at the NBRP B. subtilis, Japan
BKE19620 (ldcB::erm  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CATATTACATTACCTTCCTT,  downstream forward: _UP4_TAAAATGAAAAGCCCGCTTA
BKK19620 (ldcB::kan  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CATATTACATTACCTTCCTT,  downstream forward: _UP4_TAAAATGAAAAGCCCGCTTA

References

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