kdgK

168

2-dehydro-3-deoxygluconokinase

Locus: BSU_22110

Molecular weight: 34.88 kDa

Isoelectric point: 5.68

Protein length: 324 aa

Gene length: 975 bp

Function: utilization of galacturonic acid

Product: 2-dehydro-3-deoxygluconokinase

Essential: no

E.C.: 2.7.1.45

Synonyms: kdgK

Genomic Context

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List of homologs in different organisms, belongs to COG0524 (Galperin et al., 2021)

Gene

Coordinates: 2,323,601  2,324,575

The protein

Catalyzed reaction/ biological activity

• 2-dehydro-3-deoxy-D-gluconate + ATP --> 2-dehydro-3-deoxy-6-phospho-D-gluconate + ADP + H⁺ (according to UniProt)

Protein family

• carbohydrate kinase PfkB family (according to UniProt)

Structure

• 4DU5 (PDB) (from Polaromonas sp., 49% identity)
• P50845 (AlphaFold)

Localization

• cytoplasm (according to Swiss-Prot)

Expression and Regulation

Operons

• Genes: kdgR-kdgK-kdgA-kdgT

  Description: PubMed

  Regulation

  • induced by galacturonate (KdgR) PubMed

  Regulatory mechanism

  • KdgR: repression, PubMed, in kdgR regulon
  • CcpA: repression, PubMed, in ccpA regulon

  Sigma factors

  • SigA: sigma factor, PubMed, in sigA regulon

Biological materials

Mutant

• BKE22110 (kdgK::erm  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CATCTCTTCCCAACCTCCTC,  downstream forward: _UP4_CACCAAAAGAAAGGGGATTA
• BKK22110 (kdgK::kan  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CATCTCTTCCCAACCTCCTC,  downstream forward: _UP4_CACCAAAAGAAAGGGGATTA

References

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