ispG

168

similar to peptidoglycan acetylation, 1-hydroxy-2-methyl-2-(E)-butenyl-4-diphosphate synthase, 6th step in the MEP pathway of isoprenoid biosynthesis

Locus: BSU_25070

Molecular weight: 40.42 kDa

Isoelectric point: 6.8

Protein length: 377 aa

Gene length: 1134 bp

Function: MEP pathway of isoprenoid biosynthesis

Product: 1-hydroxy-2-methyl-2-(E)-butenyl-4-diphosphate synthase

Essential: yes

E.C.: 1.17.7.3

Synonyms: ispG, yqfY

Genomic Context

Hide small RNAsZoom:

List of homologs in different organisms, belongs to COG0821 (Galperin et al., 2021)

Gene

Coordinates: 2,589,123  2,590,256

Phenotypes of a mutant

• essential PubMed

The protein

Catalyzed reaction/ biological activity

• (2E)-4-hydroxy-3-methylbut-2-enyl diphosphate + 2 H⁺ + H₂O + oxidized [flavodoxin] --> 2-C-methyl-D-erythritol 2,4-cyclic diphosphate + reduced [flavodoxin] (according to UniProt)

Protein family

• ispG family (single member, according to UniProt)

Cofactors

• Fe-S cluster PubMed

Structure

• 3NOY (PDB) (from Aquifex aeolicus, 51% identity) PubMed
• P54482 (AlphaFold)

Localization

• secreted (according to Swiss-Prot)

Expression and Regulation

Operons

• Genes: spoIIIM-ispG

  Description: PubMed

  Regulation

  • expressed early during sporulation in the mother cell (SigE, SpoIIID) PubMed

  Regulatory mechanism

  • SpoIIID: repression, PubMed, in spoIIID regulon

  Sigma factors

  • SigE: sigma factor, PubMed, in sigE regulon

References

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