iolE

168

2-keto-myo-inositol dehydratase, dehydration of 2-keto-myo-inositol (2nd reaction)

locus: BSU_39720

Molecular weight: 33.44 kDa

pI: 5.21

Protein length: 297 aa

Gene length: 894 bp

function: myo-inositol catabolism

product: 2-keto-myo-inositol dehydratase, dehydration of 2-keto-myo-inositol (2nd reaction)

essential: no

ec: 4.2.1.44

synonyms: iolE, yxdE

List of homologs in different organisms, belongs to COG1082 (Galperin et al., 2021)

Gene

Coordinates: 4,078,173 → 4,079,066

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The protein

Catalyzed reaction/ biological activity

• scyllo-inosose --> 3D-3,5/4-trihydroxycyclohexane-1,2-dione + H₂O (according to UniProt)

Protein family

• IolE/MocC family (single member, according to UniProt)

Structure

• [PDB|3CNY] (from ''Lactobacillus plantarum wcfs1 mutant'', 63% identity, 78% similarity)
• [AF|P42416]

Biological materials

Mutant

• MGNA-B774 (iolE::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/1773 NBRP B. subtilis, Japan]
• BKE39720 (Δ[gene|C31AF6C8050D453D9011B0793403791F1851BA69|iolE]::erm  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE39720 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_TTTGCCCATCTATGGTGCCT,  downstream forward: _UP4_CTGGCTTAATGGGGAATGAA
• BKK39720 (Δ[gene|C31AF6C8050D453D9011B0793403791F1851BA69|iolE]::kan  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK39720 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_TTTGCCCATCTATGGTGCCT,  downstream forward: _UP4_CTGGCTTAATGGGGAATGAA

labs

• [wiki|Yasutaro Fujita], University of Fukuyama, Japan
• [[Ken-ichi Yoshida]], Kobe University, Japan

References

• 9226270,14993306,9887260,18310071,14993306