ilvB

168

acetolactate synthase (large subunit)

Locus: BSU_28310

Molecular weight: 62.42 kDa

Isoelectric point: 5.18

Protein length: 574 aa

Gene length: 1725 bp

Function: biosynthesis of branched-chain amino acids

Product: acetolactate synthase (large subunit)

Essential: no

E.C.: 2.2.1.6

Synonyms: ilvB

Genomic Context

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List of homologs in different organisms, belongs to COG0028 (Galperin et al., 2021)

Gene

Coordinates: 2,895,248 → 2,896,972

The protein

Catalyzed reaction/ biological activity

• H⁺ + 2 pyruvate --> (2S)-2-acetolactate + CO₂ (according to UniProt)

Protein family

• TPP enzyme family (according to UniProt)

Cofactors

• FAD (according to UniProt)
• TPP (according to UniProt)

Structure

• 4RJJ (PDB) PubMed
• 1N53 (PDB) (T box RNA)
• P37251 (AlphaFold)

Modification

• phosphorylated on Arg-297 PubMed

Paralogous protein(s)

• YdaP, AlsS

Additional information

• subject to Clp-dependent proteolysis upon glucose starvation PubMed

Expression and Regulation

Operons

• Genes: ilvB-ilvH-ilvC-leuA-leuB-leuC-leuD

  Description: PubMed

  Regulation

  • expression is stimulated in the presence of glucose (CcpA) PubMed

  Regulatory mechanism

  • T-box: RNA switch, via tRNA controlled RNA switch, repression by BCAA, in T-box
  • TnrA: repression, PubMed, in tnrA regulon
  • CodY: repression, PubMed PubMed, in codY regulon
  • CcpA: activation, PubMed, in ccpA regulon

  Sigma factors

  • SigA: sigma factor, PubMed, in sigA regulon

  Additional information

  • An antisense RNA is predicted for leuA PubMed
  • the T-box RNA is degraded by RNase Y PubMed

Biological materials

Mutant

• GP3927 (ΔilvB::aphA3 trpC2), available in Jörg Stülke's lab
• GP324, ilvB under control of pXyl (cat), available in Jörg Stülke's lab
• BKE28310 (ΔilvB::erm  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CATTTTAGTTCCTCCTTTTG,  downstream forward: _UP4_CATGAAATGGTGGGGGTGAA
• BKK28310 (ΔilvB::kan  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CATTTTAGTTCCTCCTTTTG,  downstream forward: _UP4_CATGAAATGGTGGGGGTGAA

Expression vectors

• pGP2886: expression of Strep-ilvB by pGP380 in B. subtilis suitable for SPINE, available in Jörg Stülke's lab

LacZ fusion

• pGP520 and pGP524 (both in pAC5), pGP523 (in pAC6), all are available in Jörg Stülke's lab

References

Reviews

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Original Publications

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