ileS

168

isoleucyl-tRNA synthetase

Locus: BSU_15430

Molecular weight: 104.58 kDa

Isoelectric point: 5.19

Protein length: 921 aa

Gene length: 2766 bp

Function: translation

Product: isoleucyl-tRNA synthetase

Essential: yes

E.C.: 6.1.1.5

Synonyms: ileS

Genomic Context

Hide small RNAsZoom:

List of homologs in different organisms, belongs to COG0060 (Galperin et al., 2021)

Gene

Coordinates: 1,613,357  1,616,122

Phenotypes of a mutant

• essential PubMed

The protein

Catalyzed reaction/ biological activity

• ATP + L-isoleucine + tRNA^Ile --> AMP + diphosphate + L-isoleucyl-tRNA^Ile (according to UniProt)

Protein family

• Class-I aminoacyl-tRNA synthetase family (according to UniProt)

Structure

• 1QU2 (PDB) (from Staphylococcus aureus, 61% identity) PubMed
• Q45477 (AlphaFold)

Localization

• cytoplasm (according to Swiss-Prot)

Additional information

• subject to Clp-dependent proteolysis upon glucose starvation PubMed

Expression and Regulation

Operons

• Genes: ileS

  Description: PubMed

  Regulation

  • induced by isoleucine limitation (T-box) PubMed
  • the T-box RNA is degraded by RNase Y PubMed

  Regulatory mechanism

  • T-box: RNA switch, PubMed, in T-box

References

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