htpX
168
stress-responsive membrane protease
locus
BSU_13490
Molecular weight
32.71 kDa
pI
10.16
function
quality control of membrane proteins
product
membrane protease
essential
no
synonyms
htpX, ykrL
Outlinks
Genomic Context
Categories containing this gene/protein
List of homologs in different organisms, belongs to COG0501 (Galperin et al., 2021)
This gene is a member of the following regulons
Gene
Coordinates
1,414,997 1,415,893
Phenotypes of a mutant
increased sensitivity to membrane protein overproduction [Pubmed|22447908]
increased sensitivity to dissipation of the membrane potential by the addition of valinomycin [Pubmed|22447908]
Advertisement
The protein
Protein family
Peptidase M48B family (with [protein|4B54FF77FD5B6E22D6EF3DBECD9B84C0B5A60E18|yhfN], according to UniProt)
Structure
[PDB|3CQB] (from Vibrio parahaemolyticus, corresponds to aa 71 ... 173, 45% identity)
[AF|O31657]
[wiki|Localization]
cell membrane (according to UniProt)
Expression and Regulation
Operons
genes
[gene|62B6875301B458F04E3F65717AD3A5925A9C4973|htpX]
description
[Pubmed|22383849]
regulation
expression is increased due to mutations in ''[protein|EA6790EF30D3DDB9670FE52DFD2C5083AB6A48E1|resE]'', ''[protein|495721E4B8BF6FEC01E62E86339560F90776EED1|resB]'', ''[protein|81429F7802CD4B1F7DFDB6C900D7DA5ECA6B9F3F|sdhC]'', and'' [protein|0E1FAF4B3A967CC3882B3DDB3CA2D01FD70A0F7E|sdhA]'' [Pubmed|22447908]
regulatory mechanism
[protein|1508770D5BCA5739D13E3ABD11CC5C103C07FF25|rok]: repression, [Pubmed|22447908], in [regulon|protein:1508770D5BCA5739D13E3ABD11CC5C103C07FF25|rok regulon]
additional information
the mRNA is cleaved by [protein|BAB297D18F94FFA67B8D12A684AB4D1BCDFB4981|RNase III] [PubMed|26883633]
Biological materials
Mutant
MGNA-B316 (ykrL::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/1315 NBRP B. subtilis, Japan]
BKE13490 ([gene|62B6875301B458F04E3F65717AD3A5925A9C4973|htpX]::erm trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE13490 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATAACAACCTCCGTTATTT, downstream forward: _UP4_TAATACAAACACATTGTTCC
BKK13490 ([gene|62B6875301B458F04E3F65717AD3A5925A9C4973|htpX]::kan trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK13490 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATAACAACCTCCGTTATTT, downstream forward: _UP4_TAATACAAACACATTGTTCC
References
Reviews
Original Publications
Page visits: 2541
Time of last update: 2024-04-12 21:48:24
Author of last update: Jstuelk