hmp

168

flavohemoglobin, involved in resistance to nitric oxide (NO), essential for long-term survival under anaerobic conditions

locus: BSU_13040

Molecular weight: 44.57 kDa

pI: 5.7

Protein length: 399 aa

Gene length: 1200 bp

function: resistance to NO

product: flavohemoglobin

essential: no

synonyms: hmp, ykiA

List of homologs in different organisms, belongs to COG1018 (Galperin et al., 2021)

Gene

Coordinates: 1,372,792 → 1,373,991

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The protein

Catalyzed reaction/ biological activity

• NADPH + 2 nitric oxide + 2 O₂ --> H⁺ + NADP⁺ + 2 nitrate (according to UniProt)
• NADH + 2 nitric oxide + 2 O₂ --> H⁺ + NAD⁺ + 2 nitrate (according to UniProt)

Protein family

• globin family (single member, according to UniProt)
• C-terminal part: flavoprotein pyridine nucleotide cytochrome reductase family (single member, according to UniProt)

[wiki|Domains]

• [wiki|FAD-binding FR-type domain] (aa 152-255) (according to UniProt)

[wiki|Cofactors]

• FAD (according to UniProt)

Structure

• [PDB|1CQX] (from Ralstonia eutropha, 47% identity) [pubmed|8557026]
• [AF|P49852]

Expression and Regulation

Operons

• genes: [gene|E272A68088B689873CE3269CFDD2DD830F3AB3E4|hmp]

  description: [Pubmed|8682784]

  regulation

  • expressed under anaerobic conditions ([protein|search|ResD]) [Pubmed|10972836]

  regulatory mechanism

  • [protein|1E829D639BADDC6BDC9B5FB4C97A632A53FDDB24|resD]: activation, [Pubmed|10972836], in [regulon|protein:1E829D639BADDC6BDC9B5FB4C97A632A53FDDB24|resD regulon]
  • [protein|EC6697D5D945B7E5083AFED9218748763C443278|nsrR]: repression, [Pubmed|16885456], in [regulon|protein:EC6697D5D945B7E5083AFED9218748763C443278|nsrR regulon]

  sigma factors

  • [protein|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|sigA]: sigma factor, [Pubmed|8682784], in [regulon|protein:360F48D576DE950DF79C1A2677B7A35A8D8CC30C|sigA regulon]

• genes: [gene|E272A68088B689873CE3269CFDD2DD830F3AB3E4|hmp]-[gene|5688E6CB20A55856F4A4D893CED3D54BA21209AE|ykjA]

  description: [Pubmed|22383849]

  regulation

  • expressed under anaerobic conditions ([protein|search|ResD]) [Pubmed|10972836]

Biological materials

Mutant

• BKE13040 (Δ[gene|E272A68088B689873CE3269CFDD2DD830F3AB3E4|hmp]::erm  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE13040 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_CATTTTATAAACAATCCTTT,  downstream forward: _UP4_TGAACACATACATGAAACTC
• BKK13040 (Δ[gene|E272A68088B689873CE3269CFDD2DD830F3AB3E4|hmp]::kan  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK13040 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_CATTTTATAAACAATCCTTT,  downstream forward: _UP4_TGAACACATACATGAAACTC

References

• 8682784,15231799,24214949,10972836,16885456,18487332,16923910,22383849,28439033,8557026