hmoA

168

heme monooxygenase

Locus: BSU_07150

Molecular weight: 14.57 kDa

Isoelectric point: 9.13

Protein length: 125 aa

Gene length: 378 bp

Function: degradation of heme

Product: heme monooxygenase

Essential: no

Synonyms: hmoA, yetG

Genomic Context

Hide small RNAsZoom:

Categories containing this gene/protein

Cellular processes, Homeostasis, Acquisition of iron, Acquisition of iron / Other

Metabolism, Additional metabolic pathways, Iron metabolism, Acquisition of iron / Other

List of homologs in different organisms, belongs to COG2329 (Galperin et al., 2021)

This gene is a member of the following regulons

Fur regulon

Gene

Coordinates: 785,113  785,490

The protein

Catalyzed reaction/ biological activity

binds hemin in vitro with ~1:1 stoichiometry and degrades hemin in the presence of an electron donor PubMed
heme b + 3 O₂ + 3 reduced [NADPH—hemoprotein reductase] --> biliverdin IXα + CO + Fe²⁺  + H⁺ + 3 H₂O + 3 oxidized [NADPH—hemoprotein reductase] (according to UniProt)

Protein family

antibiotic biosynthesis monooxygenase family (with HmoB, according to UniProt)

Domains

ABM domain (aa 2-95) (according to UniProt)

Structure

1TZ0 (PDB) (from B. cereus, 57% identity)
O31534 (AlphaFold)

Localization

cytoplasm (according to Swiss-Prot)

Expression and Regulation

Operons

Genes: hmoA

Description: (based on genomic context) null

Regulation

induced by iron limitation (Fur) PubMed

Regulatory mechanism

Fur: repression, PubMed, in fur regulon

Biological materials

Mutant

MGNA-B458 (yetG::erm), available at the NBRP B. subtilis, Japan
BKE07150 (hmoA::erm  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CATTTGTCTCACTCCAATCA,  downstream forward: _UP4_TAAAAAGCTTGCGCCTTGCT
BKK07150 (hmoA::kan  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CATTTGTCTCACTCCAATCA,  downstream forward: _UP4_TAAAAAGCTTGCGCCTTGCT

References

Loading