hisJ

168

histidinol phosphate phosphatase

Locus: BSU_29620

Molecular weight: 30.33 kDa

Isoelectric point: 5.06

Protein length: 268 aa

Gene length: 807 bp

Function: biosynthesis of histidine

Product: histidinol phosphate phosphatase

Essential: no

E.C.: 3.1.3.15

Synonyms: hisJ, ytvP

Genomic Context

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Categories containing this gene/protein

Metabolism, Amino acid/ nitrogen metabolism, Biosynthesis/ acquisition of amino acids, Biosynthesis/ acquisition of histidine

List of homologs in different organisms, belongs to COG1387 (Galperin et al., 2021)

Gene

Coordinates: 3,031,614 → 3,032,420

Phenotypes of a mutant

auxotroph for histidine

The protein

Catalyzed reaction/ biological activity

H₂O + L-histidinol phosphate --> L-histidinol + phosphate (according to UniProt)

Protein family

PHP hydrolase family (single member, according to UniProt)

Structure

3DCP (PDB) (from Listeria monocytogenes, 35% identity)
O34411 (AlphaFold)

Expression and Regulation

Operons

Genes: hisJ-S1129-S1130-S1131-S1132-ytsP

Description: PubMed

Biological materials

Mutant

MGNA-A427 (ytvP::erm), available at the NBRP B. subtilis, Japan
BKE29620 (ΔhisJ::erm  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CATTGATGTCACCCCATGAA,  downstream forward: _UP4_TATGAGGCGTTTTTGGAACG
BKK29620 (ΔhisJ::kan  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CATTGATGTCACCCCATGAA,  downstream forward: _UP4_TATGAGGCGTTTTTGGAACG

Labs working on this gene/protein

Stephane Aymerich, Microbiology and Molecular Genetics, INRA Paris-Grignon, France

References

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