gmuE

168

mannose kinase

Locus: BSU_05860

Molecular weight: 32.29 kDa

Isoelectric point: 5.01

Protein length: 299 aa

Gene length: 900 bp

Function: glucomannan utilization

Product: mannose kinase

Essential: no

E.C.: 2.7.1.4

Synonyms: gmuE, ydhR

Genomic Context

Hide small RNAsZoom:

List of homologs in different organisms, belongs to COG1940 (Galperin et al., 2021)

Gene

Coordinates: 630,912 → 631,811

The protein

Catalyzed reaction/ biological activity

• ATP + D-fructose --> ADP + D-fructose 6-phosphate + H⁺ (according to UniProt)

Protein family

• ROK (NagC/XylR) family (with GlcK and XylR, according to UniProt)

Structure

• 1XC3 (PDB) PubMed
• O05510 (AlphaFold)

Expression and Regulation

Operons

• Genes: gmuB-gmuA-gmuC-gmuD-gmuR-gmuE-gmuF-gmuG

  Description: PubMed

  Regulation

  • induced by cellobiose (GmuR) PubMed

  Regulatory mechanism

  • GmuR: repression, PubMed, in gmuR regulon
  • CcpA: repression, PubMed, in ccpA regulon
  • AbrB: activation, PubMed, in abrB regulon

  Sigma factors

  • SigA: sigma factor, PubMed, in sigA regulon

Biological materials

Mutant

• MGNA-C193 (ydhR::erm), available at the NBRP B. subtilis, Japan
• BKE05860 (ΔgmuE::erm  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CATGCAGATGGCCTCCTTGT,  downstream forward: _UP4_GCAGCATCCGGGGAGGTGCG
• BKK05860 (ΔgmuE::kan  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CATGCAGATGGCCTCCTTGT,  downstream forward: _UP4_GCAGCATCCGGGGAGGTGCG

References

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