gmk

168

guanylate kinase (GMP:dATP, dGMP:ATP)

Locus: BSU_15680

Molecular weight: 23.07 kDa

Isoelectric point: 4.62

Protein length: 204 aa

Gene length: 615 bp

Function: GTP biosynthesis

Product: guanylate kinase (GMP:dATP, dGMP:ATP)

Essential: yes

E.C.: 2.7.4.8

Synonyms: gmk, yloD

Genomic Context

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List of homologs in different organisms, belongs to COG0194 (Galperin et al., 2021)

Gene

Coordinates: 1,641,949  1,642,563

Phenotypes of a mutant

• essential PubMed
• mutations of gmk resulting in reduced activity of Gmk can overcome the toxicity of guanosine to a rel sasA sasB triple mutant PubMed

The protein

Catalyzed reaction/ biological activity

• ATP + GMP --> ADP + GDP (according to UniProt)

Protein family

• guanylate kinase family (single member, according to UniProt)

Domains

• Guanylate kinase-like domain (aa 5-184) (according to UniProt)

Structure

• 1S4Q (PDB) (from Mycobacterium tuberculosis h37rv, 39% identity, 60% similarity)
• 4QRH (from Staphylococcus aureus, complex with pppGpp) PubMed
• O34328 (AlphaFold)

Modification

• phosphorylated on Arg-134 and/or Arg-149PubMed

Effectors of protein activity

• inhibition of enzymatic activity by (p)ppGpp during the stringent responsePubMed

Localization

• cytoplasm (according to Swiss-Prot)

Expression and Regulation

Operons

• Genes: yloC-remA-gmk-yloH

  Description

  • PubMed

  Regulation

  • expressed during vegetative growth and early during sporulation in the forespore (SigF) PubMed

  Sigma factors

  • SigF: sigma factor, PubMed, in sigF regulon

• Genes: yloC-remA-gmk-yloH-coaBC-priA-defA-fmt-yloM-yloN

  Description

  • PubMed

• Genes: yloB-yloC-remA-gmk-yloH

  Description

  • PubMed

  Regulation

  • expressed during sporulation in the mother cell PubMed

References

Reviews

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Original Publications

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