glyA

168

serine hydroxymethyltransferase

Locus: BSU_36900

Molecular weight: 45.33 kDa

Isoelectric point: 5.47

Protein length: 415 aa

Gene length: 1245 bp

Function: biosynthesis of glycine

Product: serine hydroxymethyltransferase

Essential: no

E.C.: 2.1.2.1

Synonyms: glyA, glyC, ipc-34d

Genomic Context

Hide small RNAsZoom:

List of homologs in different organisms, belongs to COG0112 (Galperin et al., 2021)

Gene

Coordinates: 3,789,190 3,790,437

Phenotypes of a mutant

• essential PubMed, non-essential according to PubMed
• inactivation of ''glyA suppresses the synthetic lethality of the defA defB'' double mutant PubMed
• poor growth PubMed
• poorly transformable PubMed

The protein

Catalyzed reaction/ biological activity

• (6R)-5,10-methylene-5,6,7,8-tetrahydrofolate + glycine + H₂O --> (6S)-5,6,7,8-tetrahydrofolate + L-serine (according to UniProt)
• generation of 5-formyl-THF as a side product

Protein family

• SHMT family (single member, according to UniProt)

Cofactors

• PLP (according to UniProt)

Structure

• 2VGU (PDB) (complex with L-serine, Geobacillus stearothermophilus), 2VI8 (PDB) (Geobacillus stearothermophilus)
• P39148 (AlphaFold)

Modification

• phosphorylated on ser/ thr/ tyr PubMed, PubMed

Localization

• cytoplasm (according to Swiss-Prot)

Additional information

• belongs to the 100 most abundant proteins PubMed

Expression and Regulation

Operons

• Genes: glyA

  Description: PubMed

  Regulation

  • induced by glycine limitation ([regulon|T-box|]) PubMed

  Regulatory mechanism

  • PurR: repression, (molecular inducer: PRPP) PubMed, in purR regulon
  • T-box: RNA switch, in T-box

  Sigma factors

  • SigA: sigma factor, PubMed, in sigA regulon

Biological materials

Mutant

• BKE36900 (''glyA::ermC'') (available in the BGSC and in Jrg Stlke's lab) PubMed
• BKE36900 (glyA::erm trpC2) available at BGSC, PubMed, upstream reverse: _UP1_CATCAGCGAGATCCTCTCCT, downstream forward: _UP4_TAAGATCCTAAAACCCGCTT
• BKK36900 (glyA::kan trpC2) available at BGSC, PubMed, upstream reverse: _UP1_CATCAGCGAGATCCTCTCCT, downstream forward: _UP4_TAAGATCCTAAAACCCGCTT

References

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