glpQ

168

glycerolphosphate diester phosphodiesterase, degrades wall teichoic acid during phosphate starvation

locus: BSU_02130

Molecular weight: 32.81 kDa

pI: 9.26

Protein length: 293 aa

Gene length: 882 bp

function: glycerol-3-phosphate utilization, degradation of wall teichoic acid during phosphate starvation

product: glycerolphosphate diester phosphodiesterase

essential: no

ec: 3.1.4.46

synonyms: glpQ, ybeD

List of homologs in different organisms, belongs to COG0584 (Galperin et al., 2021)

Gene

Coordinates: 233,014 → 233,895

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The protein

Catalyzed reaction/ biological activity

• exohydrolytic activity on wall teichoic acid, acts in concert with [protein|597771E2E8EC31ED9B2CC8C0E4D888DEEA80F689|phoD] [Pubmed|27780866]
• removes glycerolphosphates from the free end of the unsubstituted wall teichoic acid up to the peptidoglycan linker [pubmed|32047114]
• sn-glycero-3-phosphoester + H₂O --> alcohol + H⁺ + sn-glycerol 3-phosphate (according to UniProt)

Protein family

• glycerophosphoryl diester phosphodiesterase family (with [protein|04CACAF4F786090AB67D7C606C944F07B7A7FE55|yhdW], according to UniProt)

[wiki|Domains]

• GP-PDE domain (aa 38-290) (according to UniProt)

Structure

• [PDB|5T9B] [Pubmed|27780866]
• [AF|P37965]

Paralogous protein(s)

• [protein|04CACAF4F786090AB67D7C606C944F07B7A7FE55|yhdW], [protein|AF37A0E08AAAA9159DD1F0183A61713EF582E830|yqiK]

[wiki|Localization]

• extracellular (signal peptide) [Pubmed|18957862]

Biological materials

Mutant

• BKE02130 (Δ[gene|E9BEF368F8E55888AD4849E8906E517831A882DF|glpQ]::erm  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE02130 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_CATGATTTCTCCTCCTTATA,  downstream forward: _UP4_TAAAAGCAAAAAACCTTGCG
• BKK02130 (Δ[gene|E9BEF368F8E55888AD4849E8906E517831A882DF|glpQ]::kan  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK02130 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_CATGATTTCTCCTCCTTATA,  downstream forward: _UP4_TAAAAGCAAAAAACCTTGCG

References

Reviews

• 24373430

Original Publications

• 8012593,10913081,18214974,18957862,27780866,32047114