glnR

168

transcriptional repressor ([wiki|MerR family]) of the [gene|641C4BDD9702804642E1753A9C779E80FABB3919|glnR]-[gene|68F4E792B99F86AE9E8F06D2200E128937331F5D|glnA] operon

locus: BSU_17450

Molecular weight: 15.69 kDa

pI: 9.73

Protein length: 135 aa

Gene length: 408 bp

function: regulation of glutamine synthesis

product: transcription repressor ([wiki|MerR family])

essential: no

synonyms: glnR

List of homologs in different organisms, belongs to COG0789 (Galperin et al., 2021)

Gene

Coordinates: 1,877,959  1,878,366

Advertisement

The protein

Protein family

• [wiki|MerR family]

[wiki|Domains]

• [wiki|HTH merR-type domain] (aa 11-79) (according to UniProt)

[wiki|Cofactors]

• feedback-inhibited [protein|68F4E792B99F86AE9E8F06D2200E128937331F5D|glnA] [Pubmed|18195355]

Structure

• [PDB|4R4E] (the GlnR-DNA complex) [Pubmed|25691471]
• [PDB|7TFC] (the [protein|68F4E792B99F86AE9E8F06D2200E128937331F5D|glnA]-[protein|641C4BDD9702804642E1753A9C779E80FABB3919|glnR] (C tail peptide) complex) [pubmed|35778410]
• [AF|P37582]

Effectors of protein activity

• activity is enhanced upon interaction of the C-terminal domain with [protein|68F4E792B99F86AE9E8F06D2200E128937331F5D|glnA] [Pubmed|18331450]

Expression and Regulation

additional information

• the mRNA is cleaved by [protein|BAB297D18F94FFA67B8D12A684AB4D1BCDFB4981|RNase III] [PubMed|26883633]

Biological materials

Mutant

• BP148 (del(''[gene|641C4BDD9702804642E1753A9C779E80FABB3919|glnR]-[gene|68F4E792B99F86AE9E8F06D2200E128937331F5D|glnA]'')::''cat''), available in [wiki|Fabian Commichau]'s lab
• BKE17450 ([gene|641C4BDD9702804642E1753A9C779E80FABB3919|glnR]::erm  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE17450 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_CATCTCAATTTCCTCCTTTT,  downstream forward: _UP4_TAATCCGTTAGCAACTGTTT
• BKK17450 ([gene|641C4BDD9702804642E1753A9C779E80FABB3919|glnR]::kan  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK17450 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_CATCTCAATTTCCTCCTTTT,  downstream forward: _UP4_TAATCCGTTAGCAACTGTTT

lacZ fusion

• pGP189 (in [wiki|pAC7]), available in [wiki|Jörg Stülke]'s lab

labs

• [wiki|Susan Fisher], Boston, USA [http://www.bumc.bu.edu/microbiology/research/susan-h-fisher-phd/ homepage]

References

Reviews

• 22625175

Original Publications

• 12374841,9287005,8799114,18195355,2573733,8636055,19233925,2906311,18331450,16547045,23535029,25691471,25755103,26883633,28835263,29794222,35778410