gerAC

168

lipoprotein, nutrient receptor, [wiki|germination] response to L-alanine, triggers premature [wiki|germination] in response to morphological defects during [wiki|sporulation]

locus: BSU_33070

Molecular weight: 42.23 kDa

pI: 7.3

Protein length: 373 aa

Gene length: 1122 bp

function: germination response to L-alanine

product: part of the nutrient-gated [protein|EEDE6A22E91992ABCA677CAB7917B7168C5CB868|gerAA]-[protein|5FF739B8C087207039BB6DC293EE77F59337ED0B|gerAB]-[protein|D937E26DF69D49B1341B37596AC6A8FBEEF8BA2E|gerAC] ion channel

essential: no

synonyms: gerAC

List of homologs in different organisms, belongs to COG5903 (Galperin et al., 2021)

Gene

Coordinates: 3,393,293 → 3,394,414

Phenotypes of a mutant

• suppresses the defects of [gene|40BCEB3648585961D22B71BBB4FB62EE4B3E360D|spoVV] mutant spores [pubmed|28605069]

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The protein

Catalyzed reaction/ biological activity

• the [protein|EEDE6A22E91992ABCA677CAB7917B7168C5CB868|gerAA]-[protein|5FF739B8C087207039BB6DC293EE77F59337ED0B|gerAB]-[protein|D937E26DF69D49B1341B37596AC6A8FBEEF8BA2E|gerAC] ion channel releases ions from the spore core in response to L-alanine to trigger [category|SW.4.2.4|Germination] [pubmed|37104613]
• triggers premature [wiki|germination] in response to morphological defects during [wiki|sporulation] [pubmed|28605069]

Protein family

• [wiki|GerABKC lipoprotein family] (according to UniProt)

[wiki|Domains]

• has an N-terminal signal sequence, lipidated on a Cys residue adjacent to the signal peptide [Pubmed|23335419]

Structure

• [PDB|3N54] ([protein|93D4D6864686E6E559E6CEFA69BAC77EEFAA1BE3|gerBC], 34% identity) [pubmed|20654628]
• [AF|P07870]

Paralogous protein(s)

• [protein|93D4D6864686E6E559E6CEFA69BAC77EEFAA1BE3|gerBC]

[wiki|Localization]

• cell membrane (according to UniProt)
• outer surface of the inner spore membrane [Pubmed|23335419,21378181]

Additional information

• 1,100 molecules are present per spore [Pubmed|23749970]

Expression and Regulation

Operons

• genes: [gene|EEDE6A22E91992ABCA677CAB7917B7168C5CB868|gerAA]-[gene|5FF739B8C087207039BB6DC293EE77F59337ED0B|gerAB]-[gene|D937E26DF69D49B1341B37596AC6A8FBEEF8BA2E|gerAC]

  description: [Pubmed|3110007]

  regulation

  • expressed during sporulation in the forespore ([protein|search|SigF], [protein|search|SigG], [wiki|SpoVT]) [Pubmed|16497325,15699190,8755877]

  regulatory mechanism

  • [protein|01EF886D3D004EA9B22AC5E75D50FF07D8631E68|spoVT]: repression, [Pubmed|8755877], in [regulon|protein:01EF886D3D004EA9B22AC5E75D50FF07D8631E68|spoVT regulon]

  sigma factors

  • [protein|5F24258282F3166B7696B9E9ABC1706E4D06C944|sigG]: sigma factor, [Pubmed|15699190], in [regulon|protein:5F24258282F3166B7696B9E9ABC1706E4D06C944|sigG regulon]
  • [protein|CFF318EE6CACDEE763FBF96A2ABB46E944679AAA|sigF]: sigma factor, [Pubmed|16497325], in [regulon|protein:CFF318EE6CACDEE763FBF96A2ABB46E944679AAA|sigF regulon]

  additional information

  • 1,100 molecules are present per spore [PubMed|23749970]

Biological materials

Mutant

• BKE33070 (Δ[gene|D937E26DF69D49B1341B37596AC6A8FBEEF8BA2E|gerAC]::erm  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE33070 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_AAACATACATAAAATCCGGA,  downstream forward: _UP4_AAAGGCGCAAACAAATAGCA
• BKK33070 (Δ[gene|D937E26DF69D49B1341B37596AC6A8FBEEF8BA2E|gerAC]::kan  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK33070 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_AAACATACATAAAATCCGGA,  downstream forward: _UP4_AAAGGCGCAAACAAATAGCA

References

Reviews

• 27337279,37270332

Original Publications

• 36815589,3110007,17573930,12670969,2110996,15774895,16497325,16352818,8755877,21378181,21378197,21696470,15126458,22327596,22493018,23335419,23396907,22343299,16740944,23749970,24752279,24916603,11418573,28605069,20654628,34780301,37104613