fdhD

168

sulphurtransferase, essential for the activity of the formate dehydrogenases [protein|E54F39E72DC4881E886159607C2B424A41DE0D01|forC1]-[protein|01C43D05A53BF4B0F505D9C9CCAAFA1AF9E94F87|forE1] and [protein|A4304213CFC152B601B2EED2B9B3A0A3AB4E6A81|forC2]-[protein|364BC3B51D8DF152C2DF31046D4F80595C75884B|forE2]

locus: BSU_36710

Molecular weight: 29.14 kDa

pI: 6.19

Protein length: 262 aa

Gene length: 789 bp

function: sulphur transfer to formate dehydrogenases [protein|E54F39E72DC4881E886159607C2B424A41DE0D01|forC1]-[protein|01C43D05A53BF4B0F505D9C9CCAAFA1AF9E94F87|forE1] and [protein|A4304213CFC152B601B2EED2B9B3A0A3AB4E6A81|forC2]-[protein|364BC3B51D8DF152C2DF31046D4F80595C75884B|forE2]

product: sulphurtransferase

essential: no

synonyms: fdhD, narQ, narAA

List of homologs in different organisms, belongs to COG1526 (Galperin et al., 2021)

Gene

Coordinates: 3,773,366  3,774,154

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The protein

Catalyzed reaction/ biological activity

• sulphur transfer to formate dehydrogenases [protein|E54F39E72DC4881E886159607C2B424A41DE0D01|forC1]-[protein|01C43D05A53BF4B0F505D9C9CCAAFA1AF9E94F87|forE1] and [protein|A4304213CFC152B601B2EED2B9B3A0A3AB4E6A81|forC2]-[protein|364BC3B51D8DF152C2DF31046D4F80595C75884B|forE2] [pubmed|34748728,22194618]

Protein family

• fdhD family (single member, according to UniProt)

Structure

• [PDB|4PDE] (from E. coli, 29% identity) [pubmed|25649206]
• [AF|P39756]

[wiki|Localization]

• cytoplasm (according to Swiss-Prot)

Expression and Regulation

Operons

• genes: [gene|095DAEED5F22A3BC46614C16CC907A2DB9E32C6E|fdhD]-[gene|19EB8E7CBC0E8D7A6429ADFFF93D1F71EFE3FF11|moaA]

  description: [Pubmed|22383849]

Biological materials

Mutant

• BKE36710 ([gene|095DAEED5F22A3BC46614C16CC907A2DB9E32C6E|fdhD]::erm  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE36710 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_CAATGTCTCTTCCCCTCCGC,  downstream forward: _UP4_AGGGAGTAGGAAGGGGCTGT
• BKK36710 ([gene|095DAEED5F22A3BC46614C16CC907A2DB9E32C6E|fdhD]::kan  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK36710 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_CAATGTCTCTTCCCCTCCGC,  downstream forward: _UP4_AGGGAGTAGGAAGGGGCTGT

References

• 7860592,25649206,34748728,22194618