fadR

168

transcriptional repressor (TetR family), regulation of fatty acid degradation

Locus: BSU_28550

Molecular weight: 21.83 kDa

Isoelectric point: 6.12

Protein length: 194 aa

Gene length: 585 bp

Function: regulation of fatty acid degradation

Product: transcriptional repressor

Essential: no

Synonyms: fadR, ysiA

Genomic Context

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Categories containing this gene/protein

Metabolism, Lipid metabolism, Utilization of lipids, Utilization of fatty acids

Information processing, Regulation of gene expression, Transcription factors and their control, Transcription factors/ other

List of homologs in different organisms, belongs to COG1309 (Galperin et al., 2021)

Gene

Coordinates: 2,917,957  2,918,541

The protein

Protein family

TetR family

Domains

HTH tetR-type domain (aa 5-65) (according to UniProt)

Structure

3WHB (PDB) PubMed
P94548 (AlphaFold)

Effectors of protein activity

long chain fatty acids act as inducers that release FadR from its operator sites PubMed

Expression and Regulation

Operons

Genes: lcfA-fadR-fadB-etfB-etfA

Description: PubMed

Regulation

induced by long chain acyl-CoA (C14 ... C20) (FadR) PubMed

Regulatory mechanism

FadR: repression, PubMed, in fadR regulon
CcpA: repression, PubMed, in ccpA regulon

Sigma factors

SigA: sigma factor, PubMed, in sigA regulon

Genes: fadR-fadB-etfB-etfA

Description: PubMed

Regulation

induced by long-chain fatty acids (FadR) PubMed

Biological materials

Mutant

MGNA-B008 (ysiA::erm), available at the NBRP B. subtilis, Japan
1A853 ( fadR::cat), PubMed, available at BGSC
BKE28550 (fadR::erm  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CAATGTTCATCGTTCCTTCC,  downstream forward: _UP4_AATAAGTAAAGGAGTTCTGT
BKK28550 (fadR::kan  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CAATGTTCATCGTTCCTTCC,  downstream forward: _UP4_AATAAGTAAAGGAGTTCTGT

References

Reviews

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Original Publications

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