epsL

168

phosphoglycosyl transferase, extracellular polysaccharide synthesis

locus: BSU_34250

Molecular weight: 22.93 kDa

pI: 9.97

Protein length: 202 aa

Gene length: 609 bp

function: biofilm formation

product: phosphoglycosyl transferase

essential: no

synonyms: epsL, yvfC

List of homologs in different organisms, belongs to COG2148 (Galperin et al., 2021)

Gene

Coordinates: 3,516,880 → 3,517,488

Advertisement

The protein

Catalyzed reaction/ biological activity

• UDP-N,N’-diacetylbacillosamine + undecaprenyl-phosphate --> undecaprenyl-PP-diNAcBac [pubmed|37650625]

Protein family

• Bacterial sugar transferase family (with [protein|search|TuaA], according to UniProt)

Structure

• [PDB|5W7L] (from Campylobacter concisus, 58% identity) [pubmed|29769739]
• [AF|P71062]

Paralogous protein(s)

• [protein|4AC72C556ED02A7DE4E1E8D69A0FC926359ACE28|tuaA/1], [protein|5790E3DB39F31017B557C2D3F428C0348A461DA6|tuaA/2]

[wiki|Localization]

• cell membrane (according to UniProt)

Biological materials

Mutant

• MGNA-A065 (yvfC::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/65 NBRP B. subtilis, Japan]
• BKE34250 (Δ[gene|D67665412B78B56752B460219445E4BEF52C3A1A|epsL]::erm  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE34250 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_CAGATCAAAAAGTCGTTTCA,  downstream forward: _UP4_ACCGGAAGCGGAGATGTGTC
• BKK34250 (Δ[gene|D67665412B78B56752B460219445E4BEF52C3A1A|epsL]::kan  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK34250 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_CAGATCAAAAAGTCGTTTCA,  downstream forward: _UP4_ACCGGAAGCGGAGATGTGTC

labs

• [wiki|Richard Losick], Harvard Univ., Cambridge, USA [http://www.mcb.harvard.edu/Losick/ homepage]

References

Reviews

• 20735481

Research papers

• 29769739,37650625

The EAR [wiki|RNA switch]

• 20374491,20230605