dnaN

168

DNA polymerase III (beta subunit), beta clamp, part of the [wiki|replisome], also involved in DNA mismatch repair, inhibitor of [protein|6740108089F13116F200C15F35C2E7561E990FEB|dnaA] oligomerization

locus: BSU_00020

Molecular weight: 41.94 kDa

pI: 4.72

Protein length: 378 aa

Gene length: 1137 bp

function: [category|SW.3.1.1|DNA replication], DNA repair

product: DNA polymerase  III (beta subunit), beta clamp

essential: yes

ec: 2.7.7.7

synonyms: dnaN, dnaG

List of homologs in different organisms, belongs to COG0592 (Galperin et al., 2021)

Gene

Coordinates: 1,939  3,075

Phenotypes of a mutant

• essential [Pubmed|12682299]

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The protein

Catalyzed reaction/ biological activity

• [protein|83E05071DEC874248D3E96B8F3A093C65939B314|dnaN] overexpression or release from the [wiki|replisome] decreases association of [protein|A039228A3805F4E7C5C2AE31C7DB0808562E88E3|yabA] with ''oriC'', increases association of [protein|6740108089F13116F200C15F35C2E7561E990FEB|dnaA] with ''oriC'' [Pubmed|21895792]
• recruits [protein|E2A8B04CD729332F9E6A13195181983F751F2F25|mutS] to the site of [wiki|DNA replication] ([wiki|replisome]) [Pubmed|23228104]
• required for bacteriophage SPP1 replication [Pubmed|23268446]
• inhibits oligomerization and helix formation of [protein|6740108089F13116F200C15F35C2E7561E990FEB|dnaA] [Pubmed|23909787]

Protein family

• Beta sliding clamp family (single member, according to UniProt)

Structure

• [PDB|4TR6] [pubmed|25170813]
• [PDB|6E8D] (complex with [protein|A86A013EDCE59D7EF84FB836A751E66C58CADF45|mutL]) [Pubmed|30916336]
• [AF|P05649]

[wiki|Localization]

• nucleoid (mid-cell spot) [Pubmed|9822387] [Pubmed|16479537]
• forms large assemblies on DNA, called clamp zones [Pubmed|21419346]
• forms foci [Pubmed|21958350]

Expression and Regulation

Operons

• genes: [gene|6740108089F13116F200C15F35C2E7561E990FEB|dnaA]-[gene|83E05071DEC874248D3E96B8F3A093C65939B314|dnaN]

  description: [Pubmed|2987848]

  regulation

  • negatively controlled by [protein|6740108089F13116F200C15F35C2E7561E990FEB|dnaA] [Pubmed|2168872] and [protein|2C54FE2ADC82FF414D732018C90649D477A925AD|spo0A] [Pubmed|14651647]

  regulatory mechanism

  • [protein|2C54FE2ADC82FF414D732018C90649D477A925AD|spo0A]: repression, [Pubmed|14651647], in [regulon|protein:2C54FE2ADC82FF414D732018C90649D477A925AD|spo0A regulon]

  sigma factors

  • [protein|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|sigA]: sigma factor, [Pubmed|2987848], in [regulon|protein:360F48D576DE950DF79C1A2677B7A35A8D8CC30C|sigA regulon]

  additional information

  • term-seq has identified a potential novel regulatory RNA element including an intrinsic transcription terminator upstream of ''dnaA'' [Pubmed|27120414]

Biological materials

two-hybrid system

• B. pertussis adenylate cyclase-based bacterial two hybrid system ([wiki|BACTH]), available in [wiki|Fabian Commichau]'s lab

GFP fusion

• [Pubmed|16942601]  [Pubmed|9822387]

References

Reviews

• 20157337,22933559,26343983,28075389

Original Publications

• 36815589,16942601,9822387,14651647,2987848,2168872,14651647,12060778,16461910,16479537,19737352,19081080,11395445,20122408,20451384,15469515,16045613,21895792,20453097,23228104,23268446,21050827,21419346,21958350,23909787,25170813,26384423,26615189,27369079,28878042,25170813,30391220,30916336

Labs working on this gene/protein

• [wiki|Philippe Noirot], Jouy-en-Josas, France [http://locus.jouy.inra.fr/cms/index.php?id=18 homepage]