dnaK

168

class I heat-shock protein (molecular chaperone)

locus: BSU_25470

Molecular weight: 65.83 kDa

pI: 4.57

Protein length: 611 aa

Gene length: 1836 bp

function: protein quality control

product: class I heat-shock protein (molecular chaperone)

essential: no

synonyms: dnaK

List of homologs in different organisms, belongs to COG0443 (Galperin et al., 2021)

Gene

Coordinates: 2,626,112 2,627,947

Phenotypes of a mutant

• reduced [wiki|protein secretion] [pubmed|32111210]
• a [gene|B6BF089BDC615205C9E4032380B1F0D5568D7C00|tig] [gene|30E0AAABB803E577D0D9FBAEF9031319CABD3D89|dnaK] double mutant exhibits an aberrant twisted and filamentous cell morphology, as well as decreased tolerance to heat and to cell wall active antibiotics and hydrolytic enzymes, indicative of defects in cell wall integrity [pubmed|36726573]

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The protein

Protein family

• heat shock protein 70 family (single member, according to UniProt)

Structure

• [PDB|2V7Y] (from ''Geobacillus kaustophilus'', 87% identity) [Pubmed|18400763]
• [PDB|4ANI] ([protein|30E0AAABB803E577D0D9FBAEF9031319CABD3D89|dnaK]-[protein|ECAB685E9038DC03FA2CC659112BB07D30DE5C8C|grpE] complex from ''Geobacillus kaustophilus'', 87% identity) [Pubmed|22544739]
• [AF|P17820]

Modification

• phosphorylated on Tyr-601 by [protein|6100EA37592A2C31BC3DE79AD7948A8D6F65F6E1|ptkA], dephosphorylated by [protein|CF73D86DB024575BE8F043A4C3996458F4262E46|ptpZ] [Pubmed|27148221,17726680]

[wiki|Localization]

• cytoplasm (according to Swiss-Prot)
• membrane-proximal (Spotty) [Pubmed|16479537]
• recruited to the membrane after ethanol stress [Pubmed|22268681]

Additional information

• belongs to the 100 most abundant proteins [PubMed|15378759]

Expression and Regulation

Operons

• genes: [gene|2E57DFB4162EF0CE759C1FC42C637E1B9E4B2F7E|hrcA]-[gene|ECAB685E9038DC03FA2CC659112BB07D30DE5C8C|grpE]-[gene|30E0AAABB803E577D0D9FBAEF9031319CABD3D89|dnaK]-[gene|D4C75B39B2DAECB9D85DAD66AAD4F923C9AEBEA7|dnaJ]-[gene|70D717806F5174447E01FF33DC8299CB025888CD|yqeT]-[gene|F8DC71B29D9C9BF4CF5124D0F01A71548306B9F9|yqeU]-[gene|6ADA5AF308D96218414EA92DFF4A57BAEAD0554B|yqeV]

  description: [Pubmed|9023197]

  regulation

  • induced by heat shock ([protein|search|HrcA]) [Pubmed|1339421]

  regulatory mechanism

  • [protein|2E57DFB4162EF0CE759C1FC42C637E1B9E4B2F7E|hrcA]: repression, [Pubmed|1339421], in [regulon|protein:2E57DFB4162EF0CE759C1FC42C637E1B9E4B2F7E|hrcA regulon]

  sigma factors

  • [protein|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|sigA]: sigma factor, [Pubmed|1339421], in [regulon|protein:360F48D576DE950DF79C1A2677B7A35A8D8CC30C|sigA regulon]

Biological materials

Mutant

• BKE25470 ([gene|30E0AAABB803E577D0D9FBAEF9031319CABD3D89|dnaK]::erm trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE25470 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CACTTGAATAACCTCCTGCT, downstream forward: _UP4_TAAGTTCTTTTTAGTGTCAG
• BKK25470 ([gene|30E0AAABB803E577D0D9FBAEF9031319CABD3D89|dnaK]::kan trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK25470 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CACTTGAATAACCTCCTGCT, downstream forward: _UP4_TAAGTTCTTTTTAGTGTCAG

two-hybrid system

• B. pertussis adenylate cyclase-based bacterial two hybrid system ([wiki|BACTH]), available in [wiki|Jrg Stlke]'s lab

References

Reviews

• 35350626

Original Publications

• 1339421,12224648,10383760,9023197,7540247,7592421,16479537,1339421,17726680,22268681,15378759,22544739,18400763,27148221,32111210,36726573,36815589