dltC

168

D-alanine carrier protein, alanylation of teichoic acid provides some resistance against positively charged antimicrobial peptides

locus: BSU_38520

Molecular weight: 8.87 kDa

pI: 3.62

Protein length: 78 aa

Gene length: 237 bp

function: biosynthesis of teichoic acid

product: D-alanine carrier protein

essential: no

ec: 6.1.1.13

synonyms: dltC, ipa-3r

List of homologs in different organisms, belongs to COG0236 (Galperin et al., 2021)

Gene

Coordinates: 3,954,987  3,955,223

Phenotypes of a mutant

• more sensitive to nisin [Pubmed|23980836]

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The protein

Catalyzed reaction/ biological activity

• receives D-alanine from [protein|795CD64B65D7CBF26E5F706C7617196CC6FCF864|dltA] [pubmed|37308592]

Protein family

• DltC family (single member, according to UniProt)

[wiki|Domains]

• [wiki|Carrier domain] (aa 1-78) (according to UniProt)

Structure

• [PDB|4BPG] [Pubmed|26193422]
• [AF|P39579]

Modification

• modified with the 4-phosphopantetheine (Ppant) group at Ser35, by [protein|200BE4802FCE3C860565B374EAADC9C1FEEF3E49|acpS] [pubmed|30283133], then further modified with a d-alanyl group by [protein|795CD64B65D7CBF26E5F706C7617196CC6FCF864|dltA], through a thioester bond [pubmed|30283133]

[wiki|Localization]

• cytoplasm (according to UniProt)

Expression and Regulation

Operons

• genes: [gene|459ED28A98F4EC7E8A9016C742DC8EB5C26B5E65|dltX]-[gene|795CD64B65D7CBF26E5F706C7617196CC6FCF864|dltA]-[gene|1B9BAEBE029BE9D4AD7E49C38B1E6DB5E8F4D4D3|dltB]-[gene|862D977CF1E185597329A1EF0915485F72482A03|dltC]-[gene|D178EC8A1FF22C99C437E01FC4843C5176564992|dltD]-[gene|2FB3F09DA60A40212FE20217A64DAE714AB807CF|dltE]

  description: [Pubmed|29794222]

  regulation

  • expression is reduced in a [protein|D04629D798E458AD538809BB2B150B7FA81D2C7E|sigV] mutant [Pubmed|21926231]
  • the mRNA is processed between [gene|459ED28A98F4EC7E8A9016C742DC8EB5C26B5E65|dltX] and [gene|795CD64B65D7CBF26E5F706C7617196CC6FCF864|dltA] by [protein|872CCB5A49C9000BD95E4B0472556D5F60F7D7A4|RNase Y], this requires the [protein|EE52DFA35B935E551871D079A9BE877DB2001A3B|ricA]-[protein|6C9A092F38739A3759793EF8B496569CD02C2E3F|ricF]-[protein|EBD15C174A03B7FCDFFE4C5DB5D86E93F1B9CAC4|ricT] complex [Pubmed|29794222]

  regulatory mechanism

  • [protein|7098319D8E88FDC2A629A3F4A21507FD7A649385|yvrHb]: activation, [Pubmed|16306698], in [regulon|protein:7098319D8E88FDC2A629A3F4A21507FD7A649385|yvrHb regulon]
  • [protein|2C54FE2ADC82FF414D732018C90649D477A925AD|spo0A]: repression, [Pubmed|7797557], in [regulon|protein:2C54FE2ADC82FF414D732018C90649D477A925AD|spo0A regulon]
  • stringent response: negative regulation, in [regulon|other_regulator:stringent response|stringent response]

  sigma factors

  • [protein|7024E4162A6D827069F882FDEACA696EBC05DD40|sigD]: sigma factor, [Pubmed|7797557], in [regulon|protein:7024E4162A6D827069F882FDEACA696EBC05DD40|sigD regulon]
  • [protein|081DF3EE9FA56209D648C7677188C61CE3AA8E41|sigM]: sigma factor, [Pubmed|18179421], in [regulon|protein:081DF3EE9FA56209D648C7677188C61CE3AA8E41|sigM regulon]
  • [protein|E77364F274A520FCCA9F5C9504E317B047BA29E6|sigX]: sigma factor, [Pubmed|14762009], in [regulon|protein:E77364F274A520FCCA9F5C9504E317B047BA29E6|sigX regulon]
  • [protein|D04629D798E458AD538809BB2B150B7FA81D2C7E|sigV]: sigma factor, [Pubmed|21926231], in [regulon|protein:D04629D798E458AD538809BB2B150B7FA81D2C7E|sigV regulon]

Biological materials

Mutant

• BKE38520 ([gene|862D977CF1E185597329A1EF0915485F72482A03|dltC]::erm  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE38520 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_AAAATCCATAGCTTTCTAAT,  downstream forward: _UP4_AATCAGCTGTCTGAGTTGAA
• BKK38520 ([gene|862D977CF1E185597329A1EF0915485F72482A03|dltC]::kan  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK38520 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_AAAATCCATAGCTTTCTAAT,  downstream forward: _UP4_AATCAGCTGTCTGAGTTGAA

References

Reviews

• 24819367

Original Publications

• 14762009,15955059,26193422,7797557,23980836,21856855,21926231,11434765,30283133,37308592