dgcP

168

diguanylate cyclase

Locus: BSU_29650

Molecular weight: 65.30 kDa

Isoelectric point: 5.7

Protein length: 579 aa

Gene length: 1740 bp

Function: synthesis of c-di-GMP

Product: diguanylate cyclase

Essential: no

Synonyms: dgcP, ytrP

Genomic Context

Hide small RNAsZoom:

List of homologs in different organisms, belongs to COG2199 (Galperin et al., 2021)

Gene

Coordinates: 3,033,696  3,035,435

The protein

Catalyzed reaction/ biological activity

• synthesis of c-di-GMP from two molecules of GTP PubMed

Domains

• contains an N-terminal GAF domain PubMed
• contains a C-terminal GGDEF domain PubMed

Structure

• O34325 (AlphaFold)

Localization

• cytoplasma, forms subcellular clusters at the periphery of exponentially growing cells PubMed

Expression and Regulation

Operons

• Genes: dgcP

  Description: PubMed

Biological materials

Mutant

• MGNA-A425 (ytrP::erm), available at the NBRP B. subtilis, Japan
• GP1591 (tet) available in Jörg Stülke's lab
• BKE29650 (dgcP::erm  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CATGTTTTTATCACCTAAAA,  downstream forward: _UP4_TAAAAAAAGCCCAAAACGAT
• BKK29650 (dgcP::kan  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CATGTTTTTATCACCTAAAA,  downstream forward: _UP4_TAAAAAAAGCCCAAAACGAT

References

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