cydD

168

ABC transporter required for expression of cytochrome bd (ATP-binding protein)

Locus: BSU_38730

Molecular weight: 64.34 kDa

Isoelectric point: 6.22

Protein length: 575 aa

Gene length: 1728 bp

Function: required for expression of cytochrome bd

Product: ABC transporter  (ATP-binding protein)

Essential: no

Synonyms: cydD, yxkN

Genomic Context

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List of homologs in different organisms, belongs to COG4987 (Galperin et al., 2021)

Gene

Coordinates: 3,973,364  3,975,091

The protein

Protein family

• ABC transporter superfamily (according to UniProt)

Domains

• has both a membrane-spanning and an ATP-binding domain PubMed
• ABC transmembrane type-1 domain (aa 18-303) (according to UniProt)
• ABC transporter domain (aa 337-570) (according to UniProt)

Structure

• 2HYD (PDB) (from Staphylococcus aureus, 28% identity) PubMed
• P94367 (AlphaFold)

Localization

• cell membrane PubMed

Expression and Regulation

Operons

• Genes: cydA-cydB-cydC-cydD

  Description: PubMed

  Regulation

  • expressed under anaerobic conditions (ResD) PubMed

  Expression

  • expression is heterogeneous PubMed

  Regulatory mechanism

  • CcpA: repression, in ccpA regulon
  • Rex: repression, PubMed, in rex regulon
  • ResD: activation, PubMed, in resD regulon

  Sigma factors

  • SigF: sigma factor, PubMed, in sigF regulon

• Genes: cydD

  Description: PubMed

  Regulation

  • expressed under anaerobic conditions (ResD) PubMed

Biological materials

Mutant

• MGNA-B748 (cydD::erm), available at the NBRP B. subtilis, Japan
• BKE38730 (cydD::erm  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CGGCAAAATCCATTCTTCTT,  downstream forward: _UP4_TAAAAGACAGCCCTAAAAGG
• BKK38730 (cydD::kan  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CGGCAAAATCCATTCTTCTT,  downstream forward: _UP4_TAAAAGACAGCCCTAAAAGG

References

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