cwlP

168

cell wall hydrolase of the SP-beta prophage region,

locus: BSU_21350

Molecular weight: 252.01 kDa

pI: 8.27

Protein length: 2285 aa

Gene length: 6858 bp

function: cell wall turnover

product: cell wall hydrolase

essential: no

synonyms: cwlP, yomI

List of homologs in different organisms, belongs to COG1196 (Galperin et al., 2021)

Gene

Coordinates: 2,249,154 → 2,256,011

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The protein

Catalyzed reaction/ biological activity

• hydrolyzes the peptidoglycan and cell wall of ''B. subtilis'' [Pubmed|20980266]

Protein family

• transglycosylase Slt family (together with [protein|BD3C36B63C5D88FDBACFB18849F3DA863F38383F|cwlQ]) (according to UniProt)

[wiki|Domains]

• contains soluble lytic transglycosylase (SLT) and peptidase M23 domains [Pubmed|20980266]
• ten [wiki|TPR repeat|tetratrichopeptide repeats] (according to UniProt)

Structure

• [AF|O31976]

[wiki|Localization]

• cell membrane (according to Swiss-Prot)

Biological materials

Mutant

• BKE21350 (Δ[gene|F43DC974DC71CB5F82DBD9B9D5ACEB49131772DB|cwlP]::erm  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE21350 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_CAAGTAAGAATCACTTCCTT,  downstream forward: _UP4_TAAGAGTCTGCAAAAGCAGA
• BKK21350 (Δ[gene|F43DC974DC71CB5F82DBD9B9D5ACEB49131772DB|cwlP]::kan  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK21350 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_CAAGTAAGAATCACTTCCTT,  downstream forward: _UP4_TAAGAGTCTGCAAAAGCAGA

References

• 36815589,20980266