cspC

168

cold shock protein

locus: BSU_05120

Molecular weight: 7.12 kDa

pI: 4.52

Protein length: 66 aa

Gene length: 201 bp

function: RNA chaperone

product: cold shock protein

essential: no

synonyms: cspC

List of homologs in different organisms, belongs to COG1278 (Galperin et al., 2021)

Gene

Coordinates: 559,264 → 559,464

Phenotypes of a mutant

• [gene|16E048FE5AF76CC771DEBBA8CB56B75A5414B3A2|cspB] [gene|D453E1E7D4256B56AFB2149731E08B104D59431B|cspC] double mutants are poorly viable at 15°C [pubmed|34361870]

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The protein

[wiki|Domains]

• [wiki|CSD domain] (aa 4-63) (according to UniProt)

Structure

• [PDB|2I5M] ([protein|16E048FE5AF76CC771DEBBA8CB56B75A5414B3A2|cspB], 71% identity) [pubmed|17481655]
• [AF|P39158]

Paralogous protein(s)

• [protein|0F89D0B03F54B283CDD99A940D43C18E6B5703BB|cspD], [protein|16E048FE5AF76CC771DEBBA8CB56B75A5414B3A2|cspB]

[wiki|Localization]

• cytoplasm (according to Swiss-Prot)

Expression and Regulation

Operons

• genes: [gene|D453E1E7D4256B56AFB2149731E08B104D59431B|cspC]

  description: [pubmed|22383849]

  expression

  • expression is increased at low temperature [Pubmed|34361870]

Biological materials

Mutant

• GP1969 (Δ[gene|D453E1E7D4256B56AFB2149731E08B104D59431B|cspC]::spec trpC2) available in [wiki|Jörg Stülke]'s lab [pubmed|34361870]
• BKE05120 (Δ[gene|D453E1E7D4256B56AFB2149731E08B104D59431B|cspC]::erm  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE05120 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_CATTTTTGTTGCCTCCTAGT,  downstream forward: _UP4_TAATCTTCAATCGTTTATAC
• BKK05120 (Δ[gene|D453E1E7D4256B56AFB2149731E08B104D59431B|cspC]::kan  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK05120 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_CATTTTTGTTGCCTCCTAGT,  downstream forward: _UP4_TAATCTTCAATCGTTTATAC

two-hybrid system

• B. pertussis adenylate cyclase-based bacterial two hybrid system (BACTH), available in [wiki|Stülke] lab

lacZ fusion

• GP1984 ([gene|DE4857E8A228F145EFB9B7AD817585258344ED1F|amyE]::(P_cspC-lacZ cat) trpC2) available in [wiki|Jörg Stülke]'s lab [pubmed|34361870]

References

• 12399512,12171653,11752341,11717297,12838604,9379903,9914312,11591689,9920884,12427936,17481655,31876031,34361870