cshB

168

DEAD-box RNA helicase, important for adaptation to low temperatures

locus: BSU_25140

Molecular weight: 49.86 kDa

pI: 9.89

Protein length: 438 aa

Gene length: 1317 bp

function: RNA helicase

product: DEAD-box RNA helicase

essential: no

synonyms: cshB, yqfR

List of homologs in different organisms, belongs to COG0513 (Galperin et al., 2021)

Gene

Coordinates: 2,594,184 → 2,595,500

Phenotypes of a mutant

• poor growth at low temperatures (16 to 20°C) [Pubmed|23175651]

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The protein

Catalyzed reaction/ biological activity

• RNA helicase
• required for [wiki|ribosome] assembly [Pubmed|23175651]
• ATP + H₂O --> ADP + H⁺ + phosphate (according to UniProt)

Protein family

• [wiki|helicase family] (according to UniProt)
• [wiki|DEAD-box RNA helicases] (according to UniProt)

[wiki|Domains]

• [wiki|Helicase ATP-binding domain] (aa 35-208) (according to UniProt)
• [wiki|Helicase C-terminal domain] (aa 235-385) (according to UniProt)

Structure

• [PDB|5IVL] ([protein|E169F9711635457EA65FA71CCE9D7FF29DD8DF02|cshA], 80% coverage, 40% identity) [Pubmed|28238534]
• [AF|P54475]

Paralogous protein(s)

• [protein|E169F9711635457EA65FA71CCE9D7FF29DD8DF02|cshA], [protein|EACD0C84AA3CE41EF05EAA3D25AA1153293EB562|deaD], [protein|0362D937D985846AEE4D0DEA57B62F915E8D35E5|yfmL]

[wiki|Localization]

• cytoplasma, colocalizes with the ribosomes [Pubmed|16352840]

Expression and Regulation

Operons

• genes: [gene|C83BD80DE0FD187D07B914F64696438ED5130107|cshB]-[gene|3C8BA04BA2BD0FEA28E99DA39BEB710CE3F5922D|nfo]

  description: [pubmed|22383849]

Biological materials

Mutant

• MGNA-C489 ([gene|C83BD80DE0FD187D07B914F64696438ED5130107|cshB]::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/2487 NBRP B. subtilis, Japan]
• GP1051 (Δ[gene|C83BD80DE0FD187D07B914F64696438ED5130107|cshB]::cat), available in [wiki|Jörg Stülke]'s lab
• BKE25140 (Δ[gene|C83BD80DE0FD187D07B914F64696438ED5130107|cshB]::erm  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE25140 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_CATTTTTCCCAGCTCCTTAA,  downstream forward: _UP4_AAGAAAAGAAAGTAGGGGAA
• BKK25140 (Δ[gene|C83BD80DE0FD187D07B914F64696438ED5130107|cshB]::kan  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK25140 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_CATTTTTCCCAGCTCCTTAA,  downstream forward: _UP4_AAGAAAAGAAAGTAGGGGAA

Expression vectors

• for expression/ purification from ''B. subtilis'' with C-terminal Strep-tag, for [wiki|SPINE], expression from the native chromomsomal site: GP1064 (spc), available in [wiki|Jörg Stülke]'s lab

two-hybrid system

• B. pertussis adenylate cyclase-based bacterial two hybrid system ([wiki|BACTH]), available in [wiki|Jörg Stülke]'s lab, [pubmed|20572937]

FLAG-tag construct

• GP1011 (spc, based on [wiki|pGP1331]), available in [wiki|Jörg Stülke]'s lab

labs

• [wiki|Mohamed Marahiel], Marburg University, Germany [http://www.uni-marburg.de/fb15/fachgebiete/bio/marahiel?language_sync=1 homepage]

References

Reviews

• 38430409

Original Publications

• 16352840,12399512,20572937,23175651,28238534,30337909