cotI

168

outer spore coat protein

locus: BSU_30920

Molecular weight: 41.09 kDa

pI: 4.99

Protein length: 357 aa

Gene length: 1074 bp

function: spore envelope

product: outer spore coat protein

essential: no

synonyms: cotI, ytaA

List of homologs in different organisms, belongs to COG5881 (Galperin et al., 2021)

Gene

Coordinates: 3,162,084 → 3,163,157

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The protein

Protein family

• cotS family (with [protein|DC99E507696A1CB23140A1CBA45DA07D1F7C7D53|cotS] and [protein|0F40291BB10DB6D7DFD94B9C7D700F4410C4EE69|yutH], according to UniProt)

Structure

• [PDB|2Q83] [pubmed|20077512]
• [AF|O34656]

[wiki|Localization]

• outer spore coat [pubmed|28870294]

Expression and Regulation

Operons

• genes: [gene|D57116C65FD11EF92FB5D2945B10A1D024AEFF22|cotI]

  description: [Pubmed|12480901]

  regulation

  • expressed late during sporulation in the mother cell ([protein|search|SigK]) [Pubmed|15699190,12480901]

  sigma factors

  • [protein|24F7FD5C7C3A68BB2760ABB8CBD8FBD65E5FF7D4|sigK]: sigma factor, [Pubmed|15699190,12480901], in [regulon|protein:24F7FD5C7C3A68BB2760ABB8CBD8FBD65E5FF7D4|sigK regulon]

Biological materials

Mutant

• MGNA-A030 (ytaA::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/30 NBRP B. subtilis, Japan]
• BKE30920 (Δ[gene|D57116C65FD11EF92FB5D2945B10A1D024AEFF22|cotI]::erm  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE30920 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_CATCCTTACCTGTTTTACTC,  downstream forward: _UP4_TAAGACCATCATTTCCCCCG
• BKK30920 (Δ[gene|D57116C65FD11EF92FB5D2945B10A1D024AEFF22|cotI]::kan  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK30920 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_CATCCTTACCTGTTTTACTC,  downstream forward: _UP4_TAAGACCATCATTTCCCCCG

References

• 12562816,15699190,12480901,20077512,28870294