coaBC

168

Coenzyme A biosynthesis bifunctional protein CoaBC; putative phosphopantothenoylcysteine synthetase/decarboxylase

locus: BSU_15700

Molecular weight: 43.82 kDa

pI: 6.01

Protein length: 406 aa

Gene length: 1221 bp

function: biosynthesis of coenzyme A

product: putative phosphopantothenoylcysteine synthetase/decarboxylase

essential: yes

ec: 4.1.1.36

synonyms: yloI

List of homologs in different organisms, belongs to COG0452 (Galperin et al., 2021)

Gene

Coordinates: 1,642,851  1,644,071

Phenotypes of a mutant

• essential [Pubmed|28189581]

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The protein

Catalyzed reaction/ biological activity

• H⁺ + N-[(R)-4-phosphopantothenoyl]-L-cysteine --> CO₂ + D-pantetheine 4'-phosphate (according to UniProt)
• (R)-4'-phosphopantothenate + CTP + L-cysteine --> CMP + diphosphate + H⁺ + N-[(R)-4-phosphopantothenoyl]-L-cysteine (according to UniProt)

Protein family

• N-terminal part: HFCD (homo-oligomeric flavin containing Cys decarboxylase) superfamily (single member, according to UniProt)
• C-terminal part: PPC synthetase family (single member, according to UniProt)

Structure

• [PDB|6TGV] (from Mycobacterium smegmatis, 38.8% identity) [pubmed|33420031]
• [PDB|5INT] (from ''Bacillus anthracis'', 60% identity in 52% C-terminal fragment)
• [AF|O35033]

Biological materials

Mutant

• MGNA-B372 (yloI::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/1371 NBRP B. subtilis, Japan]

References

• 22960854,33420031,28189581