chaA

168

H⁺/Ca²⁺ exchanger

Locus: BSU_07920

Molecular weight: 37.37 kDa

Isoelectric point: 5.21

Protein length: 351 aa

Gene length: 1056 bp

Function: calcium export

Product: calcium export via proton antiporter

Essential: no

Synonyms: chaA, yfkE

Genomic Context

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List of homologs in different organisms, belongs to COG0387 (Galperin et al., 2021)

Gene

Coordinates: 865,205  866,260

The protein

Protein family

• Ca(2+):cation antiporter (CaCA) (TC 2.A.19) family (single member, according to UniProt)

Structure

• 4KJS (PDB) PubMed
• O34840 (AlphaFold)

Localization

• cell membrane PubMed

Expression and Regulation

Operons

• Genes: chaA-yfkD

  Description: PubMed

  Regulation

  • expressed in the forespore (SigG) PubMed

  Sigma factors

  • SigB: sigma factor, PubMed, in sigB regulon
  • SigG: sigma factor, in sigG regulon

Biological materials

Mutant

• MGNA-C269 (yfkE::erm), available at the NBRP B. subtilis, Japan
• BKE07920 (chaA::erm  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CATGGTGAAATAGCTCCTTT,  downstream forward: _UP4_TAACCTGAAAAAATGATGGA
• BKK07920 (chaA::kan  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CATGGTGAAATAGCTCCTTT,  downstream forward: _UP4_TAACCTGAAAAAATGATGGA

References

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