bmrD

168

multidrug [wiki|ABC transporter ](ATP-binding protein), also involved in the signalling pathway to activate [protein|search|KinA ]at the onset of [wiki|sporulation]

locus: BSU_09720

Molecular weight: 76.12 kDa

pI: 7.08

Protein length: 673 aa

Gene length: 2022 bp

function: multiple antibiotic resistance

product: multidrug [wiki|ABC transporter ](ATP-binding protein)

essential: no

synonyms: bmrD, yheH

List of homologs in different organisms, belongs to COG1132 (Galperin et al., 2021)

Gene

Coordinates: 1,047,072  1,049,093

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The protein

Protein family

• [wiki|ABC transporter superfamily] (according to UniProt)

[wiki|Domains]

• has both a membrane-spanning and an ATP-binding domain [Pubmed|10092453]
• [wiki|ABC transmembrane type-1 domain] (aa 18-398) (according to UniProt)
• [wiki|ABC transporter domain] (aa 430-664) (according to UniProt)

Structure

• [PDB|7M33] [pubmed|34931066]
• [PDB|5MKK] (TmrAB complex from Thermus thermophilus, 38% identity) [pubmed|28069938]
• [AF|O07549]

Paralogous protein(s)

• [protein|0EB4D3E1B9AF39ECCBD79EB559FAE5BB0EE4C156|ygaD]

[wiki|Localization]

• cell membrane [Pubmed|10092453]

Expression and Regulation

Operons

• genes: [gene|E9956A958C66C4114EB974EA6F9BF30CD951034A|bmrB]-[gene|E98074149254BF56648F6CBE56E74B3D7DCD5DB3|bmrX]-[gene|67C0C9EA17EF953554D3D57CD0D285246F50C1AD|bmrC]-[gene|9B2766D2F8892AA17DB480216570C81C33355C3B|bmrD]

  description: [Pubmed|25217586]

  regulation

  • [gene|E9956A958C66C4114EB974EA6F9BF30CD951034A|bmrB]-[gene|E98074149254BF56648F6CBE56E74B3D7DCD5DB3|bmrX]-[gene|67C0C9EA17EF953554D3D57CD0D285246F50C1AD|bmrC]-[gene|9B2766D2F8892AA17DB480216570C81C33355C3B|bmrD] expression only occurs during the late-exponential and stationary growth stages ([protein|E5110D9C36E8C55AFD1419987B14A53232165F20|abrB]) [Pubmed|25217586]
  • RNA processing in the intergenic region between [gene|67C0C9EA17EF953554D3D57CD0D285246F50C1AD|bmrC] and [gene|67C0C9EA17EF953554D3D57CD0D285246F50C1AD|bmrC] (in [gene|E98074149254BF56648F6CBE56E74B3D7DCD5DB3|bmrX]) ([protein|search|Rae1]) [pubmed|37142436]

  expression

  • expression is increased upon depletion of depletion of tRNA maturation factors [gene|57A79AAF00243B5E058FA901D43AA7B55CA33F63|rnpB] or [gene|C40C5D35ED53D343C8200248FCCB010BAB388054|rnz] [pubmed|36840557]

  regulatory mechanism

  • [protein|E5110D9C36E8C55AFD1419987B14A53232165F20|abrB]: repression, [Pubmed|20817675,25217586], in [regulon|protein:E5110D9C36E8C55AFD1419987B14A53232165F20|abrB regulon]
  • [protein|E9956A958C66C4114EB974EA6F9BF30CD951034A|bmrB]: attenuation, [Pubmed|25217586], in [regulon|protein:E9956A958C66C4114EB974EA6F9BF30CD951034A|bmrB regulon]

Biological materials

Mutant

• MGNA-B491 (yheH::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/1490 NBRP B. subtilis, Japan]
• BKE09720 ([gene|9B2766D2F8892AA17DB480216570C81C33355C3B|bmrD]::erm  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE09720 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_TCTCCATAACGTTTTTCCTA,  downstream forward: _UP4_TAACGCTCAAAAACCCAAAA
• BKK09720 ([gene|9B2766D2F8892AA17DB480216570C81C33355C3B|bmrD]::kan  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK09720 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_TCTCCATAACGTTTTTCCTA,  downstream forward: _UP4_TAACGCTCAAAAACCCAAAA

References

Reviews

• 33450852

Original Publications

• 19167342,10092453,16487324,25217586,21602923,29507890,28069938,34931066,36840557