blyA

168

N-acetylmuramoyl-L-alanine amidase

locus: BSU_21410

Molecular weight: 39.47 kDa

pI: 9.94

Protein length: 367 aa

Gene length: 1104 bp

function: SPß-mediated lysis

product: N-acetylmuramoyl-L-alanine amidase

essential: no

ec: 3.5.1.28

synonyms: blyA, yomC

List of homologs in different organisms, belongs to COG5632 (Galperin et al., 2021)

Gene

Coordinates: 2,263,489 → 2,264,592

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The protein

Catalyzed reaction/ biological activity

• Hydrolyzes the link between N-acetylmuramoyl residues and L-amino acid residues in certain cell-wall glycopeptides (according to UniProt)

Protein family

• [wiki|N-acetylmuramoyl-L-alanine amidase 2 family] (according to UniProt)

[wiki|Domains]

• contains an amidase_2 domain (like [protein|C9C395A5F6A2C06EAC97F228C5C66D1628C937CF|cwlA], [protein|D5612882F1887BE342EF7072E71502382AB7289F|cwlH], [protein|50D7B97E4D3D41F74EF1942B85DE1DA4A33BEBB0|xlyA], [protein|A8AF827637B7208DFA101C9A2911BF9AB1AED08F|xlyB])
• 2 [wiki|SH3B domain]s 1 (aa 202-271, aa 298-367) (according to UniProt)
• [wiki|N-acetylmuramoyl-L-alanine amidase domain] (aa 24-158) (according to UniProt)

Structure

• [AF|O31982]

[wiki|Localization]

• secreted (according to UniProt)

Biological materials

Mutant

• BKE21410 (Δ[gene|C1EB8D05386C1B35B8002239F5247CC6AB25F786|blyA]::erm  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE21410 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_CATAAAAACATCTCCTTAAT,  downstream forward: _UP4_TAATTATATTCAATTTTCTC
• BKK21410 (Δ[gene|C1EB8D05386C1B35B8002239F5247CC6AB25F786|blyA]::kan  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK21410 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_CATAAAAACATCTCCTTAAT,  downstream forward: _UP4_TAATTATATTCAATTTTCTC

References

• 9579063,37699513